Anticorps Polyclonal de lapin anti-NFKB2,p52,p100-Specific

• Phare
• Validé par KD/KO

NFKB2,p52,p100-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15503-1-AP

Synonymes

DNA binding factor KBF2, H2TF1, LYT 10, LYT10, NFKB2, NFKB2,p100, NFKB2,p52,p100, NFKB2,p52,p100-Specific, Oncogene Lyt 10, p100, p52

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Galerie de données de validation

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  WB analysis of A431 using 15503-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15503-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 15503-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15503-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human brain using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human spleen using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human kidney using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human placenta using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human testis using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15503-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15503-1-AP (NFKB2,p52,p100-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15503-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using NFKB2,p52,p100-Specific antibody (15503-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 有核膜 (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules MCF-7, tissu cardiaque de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

15503-1-AP cible NFKB2,p52,p100-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100)
• Masse moléculaire calculée: 97 kDa
• Poids moléculaire observé: 52/100 kDa
• Numéro d’acquisition GenBank: NM_001077494
• Symbole du gène: NFKB2
• Identification du gène (NCBI): 4791
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. NFKB2 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p100 and generation of p52 by a cotranslational processing. The proteasome-mediated process ensures the production of both p52 and p100 and preserves their independent function. P52 binds to the kappa-B consensus sequence 5'-GGRNNYYCC-3', located in the enhancer region of genes involved in immune response and acute phase reactions. P52 and p100 are respectively the minor and major form; the processing of p100 being relatively poor. Isoform p49 is a subunit of the NF-kappa-B protein complex, which stimulates the HIV enhancer in synergy with p65. This antibody can bind both p52 and p100 isoforms of NFKB2.

Publications

Species	Application	Title
human	IHC	Cancers (Basel); Identification of Prognostic Markers of Gynecologic Cancers Utilizing Patient-Derived Xenograft Mouse Models.; Authors - Ha-Yeon Shin
human	IF,WB	Sci Rep; TNFR2 ligation in human T regulatory cells enhances IL2-induced cell proliferation through the non-canonical NF-κB pathway.; Authors - Jun Wang
human	WB	Pharm Biol; Simiao Qingwen Baidu decoction inhibits Epstein-Barr virus-induced B lymphoproliferative disease and lytic viral replication.; Authors - Xianhui Yang
human	WB	Mol Cell Endocrinol; Interleukin-1 increases cyclooxygenase-2 expression and prostaglandin E2 production in human granulosa-lutein cell via nuclear factor kappa B/P65 and extracellular signal-regulated kinase 1/2 signaling pathways; Authors - Shan Wan ; KD Validated
human	WB	Theranostics; Platelet membrane-coated alterbrassicene A nanoparticle inhibits calcification of the aortic valve by suppressing phosphorylation P65 NF-κB; Authors - Bingchuan Geng