Anticorps Monoclonal anti-NFKB1,p105,p50
NFKB1,p105,p50 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA
Hôte / Isotype
Mouse / IgG2a
Réactivité testée
Humain, souris
Applications
WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA
Conjugaison
Non conjugué
CloneNo.
2G1E3
N° de cat : 66992-1-Ig
Synonymes
Galerie de données de validation
Applications testées
| Résultats positifs en WB | cellules LNCaP, cellules HeLa, cellules Jurkat, cellules K-562, cellules THP-1 |
| Résultats positifs en IHC | tissu de cancer de l'estomac humain, tissu d'appendicite humain, tissu de cancer du côlon humain, tissu de cancer du sein humain, tissu de lymphome humain il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0. |
| Résultats positifs en IF/ICC | cellules HepG2, |
| Résultats positifs en FC (Intra) | cellules HepG2, |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:5000-1:50000 |
| Immunohistochimie (IHC) | IHC : 1:2000-1:8000 |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:500-1:2000 |
| Flow Cytometry (FC) (INTRA) | FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Applications publiées
| WB | See 8 publications below |
| IF | See 4 publications below |
| ChIP | See 1 publications below |
Informations sur le produit
66992-1-Ig cible NFKB1,p105,p50 dans les applications de WB, IHC, IF/ICC, FC (Intra), ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris
| Réactivité | Humain, souris |
| Réactivité citée | Humain, souris |
| Hôte / Isotype | Mouse / IgG2a |
| Clonalité | Monoclonal |
| Type | Anticorps |
| Immunogène | NFKB1,p105,p50 Protéine recombinante Ag5832 |
| Nom complet | nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 |
| Masse moléculaire calculée | 105 kDa |
| Poids moléculaire observé | 50 kDa, 105 kDa |
| Numéro d’acquisition GenBank | BC051765 |
| Symbole du gène | NFKB1 |
| Identification du gène (NCBI) | 4790 |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par protéine A |
| Tampon de stockage | PBS with 0.02% sodium azide and 50% glycerol |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
NFkB is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NFkB is activated by various intra- and extracellular stimuli such as cytokines, oxidant free radicals, ultraviolet irradiation, and bacterial or viral products. NFkB is a family of transcription factors that consists of homo- and heterodimers of NFkB1/p50 and RelA/p65 subunits, and controls a variety of cellular events including development and immune responses. All members share a conserved amino terminus domain that includes dimerization, nuclear localization, and DNA binding regions, and a carboxy terminal transactivation domain. Serines 529 and 536 in the transactivation domain of RelA/p65 are phosphorylated in response to several stimuli including phorbol ester, IL1 alpha and TNF alpha as mediated by IkB kinase and p38 MAPK. Phosphorylation of serines 529 and 536 is critical for RelA/p65 transcriptional activity. Activated NFkB translocates into the nucleus and stimulates the expression of genes involved in a wide variety of biological functions. Inappropriate activation of NFkB has been associated with a number of inflammatory diseases while persistent inhibition of NFkB leads to inappropriate immune cell development or delayed cell growth. NFKB1 appears to have dual functions such as cytoplasmic retention of attached NF-kappa-B proteins by p105 and generation of p50 by a cotranslational processing. This antibody can bind both p105 and p50 isoforms of NFKB1.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
| IHC protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
| IF protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
| FC protocol for NFKB1,p105,p50 antibody 66992-1-Ig | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Commun Biol EZH2 elicits CD8+ T-cell desert in esophageal squamous cell carcinoma via suppressing CXCL9 and dendritic cells | ||
J Ethnopharmacol Sub-chronic exposure to realgar induces liver injury via upregulating the TXNIP/NLRP3 pathway and disturbing bile acid homeostasis in mice | ||
Chem Biol Drug Des Caffeic acid-grafted chitooligosaccharides downregulate MAPK and NF-kB in RAW264.7 cells | ||
Chem Biol Interact Cordycepin inhibits glioma growth by downregulating PD-L1 expression via the NOD-like receptor/NFKB1/STAT1 axis | ||
Circ Res Spatiotemporal ATF3 Expression Determines VSMC Fate in Abdominal Aortic Aneurysm | ||
Cell Commun Signal Semaphorin 7A interacts with nuclear factor NF-kappa-B p105 via integrin β1 and mediates inflammation |
