Anticorps Polyclonal de lapin anti-NFIL3

• Phare
• Validé par KD/KO

NFIL3 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA

Conjugaison

Non conjugué

Publications

18

N° de cat : 11773-1-AP

Synonymes

NFIL3A, Interleukin-3-binding protein 1, Interleukin-3 promoter transcriptional activator, IL3BP1, E4BP4

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Galerie de données de validation

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  WB analysis using 11773-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11773-1-AP (NFIL3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 11773-1-AP

  IP result of anti-NFIL3 (IP:11773-1-AP, 4ug; Detection:11773-1-AP 1:1000) with HeLa cells lysate 1600ug.

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  IHC staining of mouse stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin cancer using 11773-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin cancer tissue slide using 11773-1-AP (NFIL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 11773-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HepG2 cells using 11773-1-AP (NFIL3 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of NK92 using 11773-1-AP

  1X10^6 NK92 were intracellularly stained with 0.4 ug Anti-Human NFIL3 (11773-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug x. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu d'estomac de souris, tissu de cancer de la peau humain, tissu d'estomac de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2
• Résultats positifs en FC (Intra): NK92, NK92 cells

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 13 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ChIP: See 5 publications below

Informations sur le produit

11773-1-AP cible NFIL3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFIL3 Protéine recombinante Ag2380
• Nom complet: nuclear factor, interleukin 3 regulated
• Masse moléculaire calculée: 462 aa, 51 kDa
• Poids moléculaire observé: 55-60 kDa
• Numéro d’acquisition GenBank: BC008197
• Symbole du gène: NFIL3
• Identification du gène (NCBI): 4783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFIL3, also named as E4BP4 and IL3BP1, belongs to the bZIP family and NFIL3 subfamily. It represses transcription from promoters with activating transcription factor (ATF) sites. NFIL3 activates transcription from the interleukin-3 promoter in T-cells. It competes for the same consensus-binding site with PAR DNA-binding factors (DBP, HLF and TEF). It is a component of the circadian clock that acts as a negative regulator for the circadian expression of PER2 oscillation in the cell-autonomous core clock. NFIL3 protects pro-B cells from programmed cell death. It is a key regulator of TH2 responses(PMID:21499227). The calculated molecular weight of NFIL3 is 51 kDa, but the modified NFIL3 is about 55-60 kDa.

Publications

Species	Application	Title
mouse	IF	Brain; Mislocated FUS is sufficient for gain-of-toxic-function amyotrophic lateral sclerosis phenotypes in mice.; Authors - Gen Shiihashi
mouse		Theranostics; The Circadian Clock Gene Bmal1 Controls Intestinal Exporter MRP2 and Drug Disposition.; Authors - Fangjun Yu
rat	WB	Neuropharmacology; Retigabine ameliorates acute stress-induced impairment of spatial memory retrieval through regulating USP2 signaling pathways in hippocampal CA1 area.; Authors - Cai Li
mouse	WB	Biochem Pharmacol; Tangeretin prevents cognitive deficit in delirium through activating RORα/γ-E4BP4 axis in mice; Authors - Min Chen
human	WB, ChIP	Front Med (Lausanne); NFIL3 Facilitates Neutrophil Autophagy, Neutrophil Extracellular Trap Formation and Inflammation During Gout via REDD1-Dependent mTOR Inactivation.; Authors - Honghu Tang
mouse	WB,chIP	Drug Metab Dispos; The Molecular Mechanism Regulating Diurnal Rhythm of Flavin-Containing Monooxygenase 5 in Mouse Liver.; Authors - Min Chen ; KD Validated