Anticorps Polyclonal de lapin anti-NFATC2

• Phare
• Validé par KD/KO

NFATC2 Polyclonal Antibody for WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 22023-1-AP

Synonymes

NFAT1, NFATP, NF-ATc2, NFAT pre-existing subunit, NFAT pre existing subunit

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Galerie de données de validation

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  WB analysis of Jurkat using 22023-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Sp2/0 using 22023-1-AP

  Sp2/0 cells were subjected to SDS PAGE followed by western blot with 22023-1-AP (NFATC2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Jurkat using 22023-1-AP

  IP result of anti-NFATC2 (IP:22023-1-AP, 4ug; Detection:22023-1-AP 1:1000) with Jurkat cells lysate 3200ug.

• 

  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lymphoma using 22023-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma tissue slide using 22023-1-AP (NFATC2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of U-251 using 22023-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U-251 cells using NFATC2 antibody (22023-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of Jurkat using 22023-1-AP

  1X10^6 Jurkat cells were intracellularly stained with 0.4 ug Anti-Human NFATC2 (22023-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules Jurkat,
• Résultats positifs en IP: cellules Jurkat
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U-251,
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 14 publications below
• IHC: See 2 publications below
• IF: See 10 publications below

Informations sur le produit

22023-1-AP cible NFATC2 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFATC2 Protéine recombinante Ag16849
• Nom complet: nuclear factor of activated T-cells, cytoplasmic, calcineurin-dependent 2
• Masse moléculaire calculée: 925 aa, 100 kDa
• Poids moléculaire observé: 135 kDa
• Numéro d’acquisition GenBank: BC136418
• Symbole du gène: NFATC2
• Identification du gène (NCBI): 4773
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of activated T-cells, cytoplasmic 2 (NFATC2), also named NFAT1, or NFATP, is a 925 amino acid protein, which is expressed in thymus, spleen, heart, testis, brain, placenta, muscle and pancreas. Cytoplasmic for the phosphorylated form and nuclear after activation that is controlled by calcineurin-mediated dephosphorylation. Rapid nuclear exit of NFATC is thought to be one mechanism by which cells distinguish between sustained and transient calcium signals. The subcellular localization of NFATC plays a key role in the regulation of gene transcription. NFATC2 plays a role in the inducible expression of cytokine genes in T-cells, especially in the induction of the IL-2, IL-3, IL-4, TNF-alpha or GM-CSF. NFATC2 promotes invasive migration through the activation of GPC6 expression and WNT5A signaling pathway. The calculated molecular weight of NFATC2 is about 97-100 kDa, but the modified NFATC2 protein is about 135 kDa.

Publications

Species	Application	Title
mouse	WB, IF	Biomaterials; Targeted regulation of lymphocytic ER stress response with an overall immunosuppression to alleviate allograft rejection.; Authors - Yingying Shi
human	WB,IF	Breast Cancer Res; Calcium-sensing stromal interaction molecule 2 upregulates nuclear factor of activated T cells 1 and transforming growth factor-β signaling to promote breast cancer metastasis.; Authors - Yutian Miao ; KD Validated
human	WB,IHC	Cell Mol Gastroenterol Hepatol; ANKRD22 Drives Rapid Proliferation of Lgr5+ Cells and Acts as a Promising Therapeutic Target in Gastric Mucosal Injury.; Authors - Jingwen Liu
	IF	Mucosal Immunol; IL-33/ST2/IL-9/IL-9R signaling disrupts ocular surface barrier in allergic inflammation.; Authors - Jiaoyue Hu
mouse	WB	J Neuroinflammation; Human PMSCs-derived small extracellular vesicles alleviate neuropathic pain through miR-26a-5p/Wnt5a in SNI mice model; Authors - Yitian Lu
mouse	WB	CNS Neurosci Ther; miR-26a-5p alleviates CFA-induced chronic inflammatory hyperalgesia through Wnt5a/CaMKII/NFAT signaling in mice; Authors - Yitian Lu