Anticorps Polyclonal de lapin anti-NFAT5

NFAT5 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 21713-1-AP

Synonymes

KIAA0827, NF AT5, NFAT5, NFATL1, NFATZ, OREBP, TonE binding protein, TONEBP

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Galerie de données de validation

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  WB analysis using 21713-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21713-1-AP (NFAT5 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human thyroid cancer using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded human thyroid cancer tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 21713-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 21713-1-AP (NFAT5 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 21713-1-AP

  Immunofluorescent analysis of HepG2 cells using 21713-1-AP (NFAT5 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules COS-7, cellules HepG2, cellules Jurkat, cellules U2OS
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cérébral de rat, tissu cérébral de souris, tissu de cancer de la thyroïde humain, tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

21713-1-AP cible NFAT5 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NFAT5 Protéine recombinante Ag16341
• Nom complet: nuclear factor of activated T-cells 5, tonicity-responsive
• Masse moléculaire calculée: 1531 aa, 166 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: BC146765
• Symbole du gène: NFAT5
• Identification du gène (NCBI): 10725
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NFAT5 (Nuclear factor of activated T-cells 5) is the member of the Rel family of transcription factors, including NF-κB and NFAT1-4, which plays central roles in inducible gene expression during the immune response (PMID: 21629436). NFAT5 also acts as a novel regulator of mammalian anti-pathogen responses, regulating TLR-induced gene expression in primary macrophages induced by Toll-like receptors (PMID: 22312110). NFAT5 is involved in the transcriptional regulation of osmoprotective and inflammatory genes (PMID: 10377394).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (10-16-2023)

Works well for western blot and IF

• Applications: Western Blot, Immunofluorescence