Anticorps Polyclonal de lapin anti-NF90/ILF3

• Phare
• Validé par KD/KO

NF90/ILF3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA

Conjugaison

Non conjugué

Publications

26

Avis (2)

N° de cat : 19887-1-AP

Synonymes

CBTF, DRBF, DRBP76, ILF3, IL-F3, M phase phosphoprotein 4, MMP4, MPHOSPH4, MPP4, NF AT 90, NF110, NF90, NFAR, NFAR 1, NFAR2, TCP110, TCP80

---

Galerie de données de validation

• 

  WB analysis using 19887-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 19887-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 19887-1-AP (NF90,ILF3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 19887-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 19887-1-AP (ILF3 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 19887-1-AP

  IP result of anti-NF90/ILF3 (IP:19887-1-AP, 3ug; Detection:19887-1-AP 1:1000) with HeLa cells lysate 1600ug.

• 

  IHC staining of human cervical cancer using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 19887-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 19887-1-AP (ILF3 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HeLa using 19887-1-AP

  Immunofluorescent analysis of Hela cells, using ILF3 antibody 19887-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

---

Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules K-562, cellules Raji, tissu splénique de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 22 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• IP: See 1 publications below
• CoIP: See 2 publications below
• RIP: See 5 publications below

Informations sur le produit

19887-1-AP cible NF90/ILF3 dans les applications de WB, IHC, IF/ICC, IP, CoIP, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF90/ILF3 Protéine recombinante Ag13707
• Nom complet: interleukin enhancer binding factor 3, 90kDa
• Masse moléculaire calculée: 894 aa, 95 kDa
• Poids moléculaire observé: 90 kDa, 110 kDa
• Numéro d’acquisition GenBank: BC064836
• Symbole du gène: ILF3
• Identification du gène (NCBI): 3609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nuclear factor of activated T cells (NFAT)is a transcription factor required for T-cell expression of interleukin-2 (IL2). NFAT binds to a sequence known as the antigen receptor response element-2 (ARRE2) in the IL2 enhancer. NFAT contains two subunits, one is ILF3. ILF3 can facilitate double-standed RNA-regulated gene expression at the level of post-transcription. It binds to and inhibits coding sequences of acid beta-glucosidase and other mRNAs and functions at the initiation phase of GCase mRNA translation, probably by inhibiting its binding to polysomes. ILF3 exists main two isoforms NFAR-1 (90 kDa) and -2 (110 kDa). NFAR-1 and -2 each contain two DRBDs in their C-terminus regions and exhibit 98% homology to one another at the amino acid level. NFAR-2 contains an extra 192 amino acids at the C-terminal region that are encoded by an extra three exons present in its corresponding transcript.

Publications

Species	Application	Title
human	WB	Cell; In vivo structural characterization of the SARS-CoV-2 RNA genome identifies host proteins vulnerable to repurposed drugs.; Authors - Lei Sun ; KD Validated
human	WB	Nat Commun; PMID: 31792210; Authors - Sunwang Xu
human	WB	EMBO J; Suppression of miR-199a maturation by HuR is crucial for hypoxia-induced glycolytic switch in hepatocellular carcinoma.; Authors - Ling-Fei Zhang
human	WB	Dev Cell; Genome-wide analysis of pseudogenes reveals HBBP1's human-specific essentiality in erythropoiesis and implication in β-thalassemia.; Authors - Yanni Ma
mouse	RIP	Cancer Res; CircFOXK2 Promotes Growth and Metastasis of Pancreatic Ductal Adenocarcinoma by Complexing with RNA Binding Proteins and Sponging MiR-942.; Authors - Chi Hin Wong
human	WB	Nucleic Acids Res; Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.; Authors - Aras Siddhesh S

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Praveen (Verified Customer) (03-13-2019)

Very Good

• Applications: Western Blot,
• Cell Tissue Type: HEK293

NF90/ILF3 Antibody Western Blot, validation ( dilution) in HEK293 (Cat no:19887-1-AP)

FH

SHUBHAM (Verified Customer) (02-25-2019)

eXCELLENT

• Applications: Western Blot,
• Cell Tissue Type: Ac16

NF90/ILF3 Antibody Western Blot, validation ( dilution) in Ac16 (Cat no:19887-1-AP)