Anticorps Polyclonal de lapin anti-NF2

NF2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 10659-1-AP

Synonymes

ACN, BANF, Merlin, Neurofibromin 2, neurofibromin 2 (merlin), NF2, SCH, Schwannomerlin, Schwannomin

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Galerie de données de validation

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  WB analysis using 10659-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 10659-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 10659-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 10659-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of NIH/3T3 using 10659-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 10659-1-AP (NF2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules NIH/3T3, tissu rénal de rat, tissu rénal de souris, tissu rénal humain

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

10659-1-AP cible NF2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NF2 Protéine recombinante Ag1013
• Nom complet: neurofibromin 2 (merlin)
• Masse moléculaire calculée: 70 kDa
• Poids moléculaire observé: 64-66 kDa
• Numéro d’acquisition GenBank: BC007279
• Symbole du gène: NF2
• Identification du gène (NCBI): 4771
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neurofibromatosis 2 (NF2) tumor suppressor protein-merlin is involved in Hippo/SWH signaling pathway that associated with tumor suppression by restricting proliferation and promoting apoptosis. It has been reported that merlin can change to phosphorylation form which inactivates merlin tumor suppressive abilities at serine 518 induced by Rac effector p21-activated kinase 2. The mutation of the NF2 tumor suppressor gene may lead to nervous system tumors, mesotheliomas and metastatic tumors.

Publications

Species	Application	Title
human	WB	J Exp Clin Cancer Res; The histone lysine acetyltransferase KAT2B inhibits cholangiocarcinoma growth: evidence for interaction with SP1 to regulate NF2-YAP signaling; Authors - Wenbo Ma