Anticorps Polyclonal de lapin anti-GNAS

• Phare
• Validé par KD/KO

GNAS Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 10150-2-AP

Synonymes

AHO, C20orf45, GNAS, GNAS complex locus, GNAS1, GPSA, GSA, GSP, NESP, PHP1A, PHP1B, POH

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Galerie de données de validation

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  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10150-2-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10150-2-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10150-2-AP (GNAS antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 10150-2-AP

  IP result of anti-GNAS (IP:10150-2-AP, 4ug; Detection:10150-2-AP 1:300) with mouse liver tissue lysate 4000ug.

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10150-2-AP (GNAS antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human colon using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human pancreas using 10150-2-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 10150-2-AP (NESP55,GNAS Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 10150-2-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using GNAS antibody (10150-2-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), 594-phalloidine (red).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HepG2, cellules MCF-7, tissu cérébral de rat, tissu cérébral de souris, tissu hépatique de rat, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de côlon humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

10150-2-AP cible GNAS dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GNAS Protéine recombinante Ag0197
• Nom complet: GNAS complex locus
• Masse moléculaire calculée: 45 kDa
• Poids moléculaire observé: 46 kDa
• Numéro d’acquisition GenBank: BC002722
• Symbole du gène: GNAS
• Identification du gène (NCBI): 2778
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Guanine nucleotide binding protein (G protein), alpha stimulating activity polypeptide 1 (GNAS1) is the ubiquitously expressed heterotrimeric G protein that couples receptors to the effector enzyme adenylyl cyclase and is required for receptor-stimulated intracellular cAMP generation. Mutations of Gs(alpha) residues involved in the GTPase reaction that lead to constitutive activation are present in endocrine tumors, fibrous dysplasia of bone, and McCune-Albright syndrome. The molecular weight of Gs(alpha) protein is about 46 kDa.

Publications

Species	Application	Title
mouse	WB	Elife; Gq activity- and β-arrestin-1 scaffolding-mediated ADGRG2/CFTR coupling are required for male fertility.; Authors - Dao-Lai Zhang
human	WB	Cell Mol Life Sci; HDAC2 and TXNL1 distinguish aneuploid from diploid colorectal cancers.; Authors - Gemoll Timo T
human	WB,IF	Cell Mol Biol Lett; GNAS promotes inflammation-related hepatocellular carcinoma progression by promoting STAT3 activation.; Authors - Hongda Ding
mouse	WB,IF	Am J Physiol Renal Physiol; Ablation of Gsa impairs renal tubule proliferation after injury via CDK2/cyclin E.; Authors - Lele Liu ; KD Validated
human	WB	Tumour Biol; A novel GNAS-mutated human induced pluripotent stem cell model for understanding GNAS-mutated tumors.; Authors - Katsuhito Watanabe ; KO Validated
human,mouse	WB	Nat Commun; Activation of melanocortin-1 receptor signaling in melanoma cells impairs T cell infiltration to dampen antitumor immunity; Authors - Yazhong Cui

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Parijat (Verified Customer) (10-16-2023)

Works well.

• Applications: Western Blot