Anticorps Polyclonal de lapin anti-NELFB

• Phare
• Validé par KD/KO

NELFB Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16418-1-AP

Synonymes

COBRA1, cofactor of BRCA1, KIAA1182, Negative elongation factor B, NELF B, NELFB

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Galerie de données de validation

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  WB analysis using 16418-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16418-1-AP

  WB result of NELFB antibody (16418-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NELFB transfected HeLa cells.

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  WB analysis of BxPC-3 using 16418-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 16418-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of BxPC-3 using 16418-1-AP

  BxPC-3 cells were subjected to SDS PAGE followed by western blot with 16418-1-AP (NELFB antibody at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 16418-1-AP

  IP result of anti-NELFB (IP:16418-1-AP, 3ug; Detection:16418-1-AP 1:500) with mouse brain tissue lysate 6000ug.

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  IHC staining of human urothelial carcinoma using 16418-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16418-1-AP (NELFB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 16418-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 16418-1-AP (NELFB antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of BxPC-3 using 16418-1-AP

  Immunofluorescent analysis of BxPC-3 cells using 16418-1-AP (NELFB antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules HepG2, cellules MCF-7, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de carcinome urothélial humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules BxPC-3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

16418-1-AP cible NELFB dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NELFB Protéine recombinante Ag9599
• Nom complet: cofactor of BRCA1
• Masse moléculaire calculée: 580aa,66 kDa; 475aa,54 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC011892
• Symbole du gène: NELFB
• Identification du gène (NCBI): 25920
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cofactor of BRCA1 (COBRA1) was first identified as a BRCA1-interacting protein and subsequently found to be the B subunit of the negative elongation factor complex (NELF-B). Cobra1 is essential for early embryogenesis (PMID: 19340312). The MW of this protein is 66 kDa, and this antibody specially recognises the 66 kDa protein.

Publications

Species	Application	Title
mouse	IF	Nature; The landscape of RNA Pol II binding reveals a stepwise transition during ZGA.; Authors - Bofeng Liu
mouse	WB	Genes Dev; High constitutive activity of a broad panel of housekeeping and tissue-specific cis-regulatory elements depends on a subset of ETS proteins.; Authors - Alessia Curina
human	WB	Nat Commun; ERK-mediated NELF-A phosphorylation promotes transcription elongation of immediate-early genes by releasing promoter-proximal pausing of RNA polymerase II; Authors - Seina Ohe
mouse	IHC	bioRxiv; RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis; Authors - Emily G Kaye
human	WB	J Biol Chem; An Essential Signaling Function of Cytoplasmic NELFB is Independent of RNA Polymerase II Pausing; Authors - Haihui Pan
mouse	IHC	Nat Commun; RNA polymerase II pausing is essential during spermatogenesis for appropriate gene expression and completion of meiosis; Authors - Emily G Kaye