Anticorps Polyclonal de lapin anti-NEK6

• Phare
• Validé par KD/KO

NEK6 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10378-1-AP

Synonymes

NEK6, NimA related protein kinase 6, Protein kinase SID6 1512, SID6 1512

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Galerie de données de validation

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  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10378-1-AP

  WB result of NEK6 antibody (10378-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEK6 transfected HEK-293 cells.

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  WB analysis of HeLa using 10378-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 10378-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 10378-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HT-1080 using 10378-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 10378-1-AP (NEK6 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HeLa using 10378-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 10378-1-AP (NEK6 antibody) at dilution of 1:50 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HT-1080
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below

Informations sur le produit

10378-1-AP cible NEK6 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEK6 Protéine recombinante Ag0266
• Nom complet: NIMA (never in mitosis gene a)-related kinase 6
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35-45 kDa
• Numéro d’acquisition GenBank: BC000101
• Symbole du gène: NEK6
• Identification du gène (NCBI): 10783
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The Aspergillus nidulans 'never in mitosis A' (NIMA) is a serine/ threonine kinase that controls initiation of mitosis, whereas its inactivation is necessary for mitotic exit. NIMA-related kinases (NEKs) are a group of protein kinases that are homologous to NIMA. Evidence suggests that NEKs perform functions similar to those of NIMA. Human NIMA-related kinase 6 (NEK6, synonym: SID6-1512) is comprised of 338 amino acids and shows both nuclear and cytoplasmic localizations in HeLa cells. NEK6 is required for mitotic progression of human cells. NEK6 is phosphorylated and activated during M phase. Inhibition of Nek6 function by either overexpression of an inactive Nek6 mutant or elimination of endogenous Nek6 by siRNA arrests cells in M phase and triggers apoptosis. Recombinant human NEK6 protein produced in insect cells effectively phosphorylates histones H1 and H3, but not casein. Thus, these results suggest that, unlike other mammalian NIMA-related kinases, NEK6 is a mitotic histone kinase which regulates chromatin condensation in mammalian cells. NEK6 transcripts are ubiquitously expressed with the highest expression found in the heart and skeletal muscle, and the hNek6 gene is localized to human chromosome 9q33-34. NEK6 has four isoforms with MW 35-40 kDa.

Publications

Species	Application	Title
human	WB	Front Mol Biosci; LncRNA FAM13A-AS1 Promotes Renal Carcinoma Tumorigenesis Through Sponging miR-141-3p to Upregulate NEK6 Expression.; Authors - Xin Jun Wang
human	WB	J Oncol; MicroRNA-323a-3p Negatively Regulates NEK6 in Colon Adenocarcinoma Cells.; Authors - Zhongshi Hong
human	WB,IF	Int J Gen Med; NEK6 Promotes the Progression of Osteosarcoma Through Activating STAT3 Signaling Pathway by Down-Regulation of miR-26a-5p; Authors - Min Zhu
human	WB	Cancers (Basel); Differential Expression of NEK Kinase Family Members in Esophageal Adenocarcinoma and Barrett's Esophagus; Authors - Lei Chen