Anticorps Polyclonal de lapin anti-NEIL3

• Phare
• Validé par KD/KO

NEIL3 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, FC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

11

Avis (1)

N° de cat : 11621-1-AP

Synonymes

DNA glycosylase FPG2, DNA glycosylase/AP lyase Neil3, Endonuclease 8 like 3, Endonuclease VIII like 3, FGP2, FPG2, hFPG2, hNEI3, Nei like protein 3, NEI3, NEIL3

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Galerie de données de validation

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  WB analysis of HEK-293 using 11621-1-AP

  WB result of NEIL3 antibody (11621-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEIL3 transfected HEK-293 cells.

• 

  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 11621-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 11621-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skin using 11621-1-AP

  mouse skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skin using 11621-1-AP

  human skin tissue were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 11621-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11621-1-AP (NEIL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules THP-1, cellules U2OS, tissu cutané de souris, tissu cutané humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 11 publications below
• IP: See 3 publications below
• FC: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

11621-1-AP cible NEIL3 dans les applications de WB, IP, FC, ChIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEIL3 Protéine recombinante Ag2200
• Nom complet: nei endonuclease VIII-like 3 (E. coli)
• Masse moléculaire calculée: 605 aa, 68 kDa
• Poids moléculaire observé: 62-68 kDa
• Numéro d’acquisition GenBank: BC025954
• Symbole du gène: NEIL3
• Identification du gène (NCBI): 55247
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEIL3 belongs to the FPG family. It is a functional DNA glycosylase in vitro and in vivo. NEIL3 plays a role in repairing FapyG in vivo. (PMID:20185759) NEIL3 has AP lyase activity specific for single-stranded DNA.(PMID:19170771). NEIL3 is specifically expressed in brain areas where neurogenesis takes place during development and that its expression is tightly regulated both temporally and spatially (PMID:19426544). Studies have shown that NEIL3 is recruited to the DSB sites through its GRF zinc finger motifs and plays an important role in MMC/cisplatin-ICL repair by promoting the HR step in FA/BRCA pathway.(PMID: 36351389).

Publications

Species	Application	Title
human	WB	Nat Commun; C/EBPβ enhances platinum resistance of ovarian cancer cells by reprogramming H3K79 methylation.; Authors - Dan Liu
human	WB	Nucleic Acids Res; Cooperation of the NEIL3 and Fanconi anemia/BRCA pathways in interstrand crosslink repair.; Authors - Niu Li ; KD Validated
human	WB	EMBO J; PrimPol-mediated repriming facilitates replication traverse of DNA interstrand crosslinks.; Authors - Daniel González-Acosta
human	WB	Sci Rep; NEIL3-mediated proteasomal degradation facilitates the repair of cisplatin-induced DNA damage in human cells; Authors - Umit Aliyaskarova
human	WB,IP	DNA Repair (Amst); Release from quiescence stimulates the expression of human NEIL3 under the control of the Ras dependent ERK-MAP kinase pathway.; Authors - Neurauter Christine Gran CG
human	WB,FC,IP,ChIP	Cell Rep; NEIL3 Repairs Telomere Damage during S Phase to Secure Chromosome Segregation at Mitosis.; Authors - Jia Zhou ; KD Validated

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Jia (Verified Customer) (09-19-2019)

Good antibody for detecting NEIL3 in human cells.

• Applications: Western Blot, Immunofluorescence, Immunoprecipitation,
• Primary Antibody Dilution: 1:100 for IP, 1:500 for WB