Anticorps Monoclonal anti-NEDD4L

NEDD4L Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

2F6H6

N° de cat : 67276-1-Ig

Synonymes

FLJ33870, hNedd4 2, KIAA0439, NEDD4 2, NEDD4.2, NEDD4L, NEDL3, RSP5

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Galerie de données de validation

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  WB analysis of Jurkat using 67276-1-Ig

  Jurkat cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HSC-T6 using 67276-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 67276-1-Ig

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 4T1 using 67276-1-Ig

  4T1 cells were subjected to SDS PAGE followed by western blot with 67276-1-Ig (NEDD4L antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  IF Staining of PC-3 using 67276-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed PC-3 cells using NEDD4L antibody (67276-1-Ig, Clone: 2F6H6 ) at dilution of 1:3000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules 4T1, cellules HSC-T6, cellules NIH/3T3
• Résultats positifs en IF: cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:3000-1:10000
• Immunofluorescence (IF): IF : 1:1500-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

67276-1-Ig cible NEDD4L dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NEDD4L Protéine recombinante Ag4565
• Nom complet: neural precursor cell expressed, developmentally down-regulated 4-like
• Masse moléculaire calculée: 854 aa, 96 kDa
• Poids moléculaire observé: 112 kDa, 125 kDa
• Numéro d’acquisition GenBank: BC032597
• Symbole du gène: NEDD4L
• Identification du gène (NCBI): 23327
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD4L(E3 ubiquitin-protein ligase NEDD4-like) is also named as KIAA0439, NEDL3, Nedd4-2. It accepts ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substratesand and is the principal ubiquitin ligase that selectively targets activated SMAD2 and SMAD3 for destruction (PMID:19917253). Nedd4L binds the PY motif of ENaC COOH terminals and catalyzes ubiquitination of the NH2 terminus of the protein for subsequent degradation (PMID:18268134). It has some isoforms produced by alternative splicing. This antibody may have cross reaction with NEDD4 due to the high homology of the antigen.