Anticorps Polyclonal de lapin anti-NEDD4

• Phare
• Validé par KD/KO

NEDD4 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, singe, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

42

Avis (1)

N° de cat : 21698-1-AP

Synonymes

KIAA0093, NEDD 4, NEDD4, NEDD4 1, RPF1

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Galerie de données de validation

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  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 21698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 21698-1-AP

  WB result of NEDD4 antibody (21698-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NEDD4 transfected HeLa cells.

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  WB analysis of HT-1080 using 21698-1-AP

  HT-1080 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 21698-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 21698-1-AP (NEDD4 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of C6 using 21698-1-AP

  IP result of anti-NEDD4 (IP:21698-1-AP, 4ug; Detection:21698-1-AP 1:500) with C6 cells lysate 1600ug.

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  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human breast cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

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  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

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  IHC staining of human liver using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 21698-1-AP (NEDD4 Antibody) at dilution of 1:50.

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 21698-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 21698-1-AP (NEDD4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 21698-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed U2OS cells using NEDD4 antibody (21698-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules C6, cellules COS-7, cellules HEK-293, cellules HeLa, cellules HT-1080, cellules Jurkat, cellules NIH/3T3
• Résultats positifs en IP: cellules C6
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de cancer du poumon humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 37 publications below
• IHC: See 4 publications below
• IF: See 10 publications below
• IP: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

21698-1-AP cible NEDD4 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, singe, souris

• Réactivité: Humain, rat, singe, souris
• Réactivité citée: rat, Humain, Lapin, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NEDD4 Protéine recombinante Ag16291
• Nom complet: neural precursor cell expressed, developmentally down-regulated 4
• Masse moléculaire calculée: 1319 aa, 149 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC152452
• Symbole du gène: NEDD4
• Identification du gène (NCBI): 4734
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NEDD4 and similar proteins discovered subsequently became a family of HECT ligases, comprising 9 human proteins including NEDD4, NEDD4-2 (NEDD4L), ITCH, SMURF1, SMURF2, WWP1, WWP2, NEDL1 and NEDL2. NEDD4 is a highly evolutionarily conserved protein from yeast to man, and was initially cloned as a highly expressed gene in the early embryonic brain. NEDD4 is frequently overexpressed in many different types of cancer, and decreased levels of NEDD4 can also be associated with cancer. It can be a potential therapeutic target for the treatment of human cancer.(PMID: 25527121). The human NEDD4 gene is located on chromosome 15q21.3 and comprises 30 exons (HGNC:7727) shown to encode a ~120 KDa protein. Otherwise there is a 75 kDa isoform in addition to full length NEDD4 (PMID: 24907641).

Publications

Species	Application	Title
human,rabbit,mouse,rat,zebrafish		Cell Metab; Microglial lipid droplet accumulation in tauopathy brain is regulated by neuronal AMPK; Authors - Yajuan Li
human	WB	J Exp Clin Cancer Res; PHB2 promotes SHIP2 ubiquitination via the E3 ligase NEDD4 to regulate AKT signaling in gastric cancer; Authors - Liang Xu
mouse	WB	Proc Natl Acad Sci U S A; Zbtb7b engages the long noncoding RNA Blnc1 to drive brown and beige fat development and thermogenesis.; Authors - Siming Li
human	WB	Theranostics; PINCH-1 promotes IGF-1 receptor expression and skin cancer progression through inhibition of the GRB10-NEDD4 complex.; Authors - Xiaoxiao Wang
human	WB	Cell Death Differ; Ubiquitin ligase CHIP regulates OTUD3 stability and suppresses tumour metastasis in lung cancer.; Authors - Pengfei Zhang
human	WB, CoIP	Clin Transl Med; Tumor-derived exosomal miR-19b-3p facilitates M2 macrophage polarization and exosomal LINC00273 secretion to promote lung adenocarcinoma metastasis via Hippo pathway.; Authors - Jing Chen

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Tongbin (Verified Customer) (08-25-2020)

This NEDD4 antibody works very well in western blots.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart