Anticorps Monoclonal anti-NDUFS3

NDUFS3 Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

1A1B3

N° de cat : 68066-1-Ig

Synonymes

CI 30kD, Complex I 30kD, NDUFS3

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Galerie de données de validation

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  WB analysis using 68066-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control.

• 

  WB analysis of pig brain using 68066-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rabbit brain using 68066-1-Ig

  rabbit brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 68066-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 68066-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 68066-1-Ig (NDUFS3 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse testis using 68066-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 68066-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 68066-1-Ig (NDUFS3 antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules Jurkat, cellules K-562, cellules LNCaP, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

68066-1-Ig cible NDUFS3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: NDUFS3 Protéine recombinante Ag7072
• Nom complet: NADH dehydrogenase (ubiquinone) Fe-S protein 3, 30kDa (NADH-coenzyme Q reductase)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 30 kDa
• Numéro d’acquisition GenBank: BC000617
• Symbole du gène: NDUFS3
• Identification du gène (NCBI): 4722
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NDUFS3(NADH dehydrogenase [ubiquinone] iron-sulfur protein 3, mitochondrial) is also named as CI-30kD and belongs to the complex I 30 kDa subunit family. It catalyzes the oxidation of NADH, with the concomitant reduction of ubiquinone and ejection of protons out of the mitochondria(PMID:10967146). NDUFS3 can be used as a mitochondrial matrix control(PMID:17344420). The full length has a transit peptide with 36 amino acids.

Publications

Species	Application	Title
human	WB	Sci Rep; Characterization of a new mutation of mitochondrial ND6 gene in hepatocellular carcinoma and its effects on respiratory complex I; Authors - Veronica Bazzani
pig	WB	Sci China Life Sci; CRISPR screening identifies protein methylation and ubiquitination modifications that modulate aflatoxin B1 cytotoxicity; Authors - Huaqiang Yang

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