Anticorps Polyclonal de lapin anti-NDUFB2

NDUFB2 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 17614-1-AP

Synonymes

AGGG, CI AGGG, Complex I AGGG, NDUFB2

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Galerie de données de validation

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  WB analysis of human heart using 17614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 17614-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 17614-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 17614-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 17614-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spleen using 17614-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 17614-1-AP (NDUFB2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17614-1-AP (NDUFB2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human heart using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human heart using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human kidney using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human liver using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human liver using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human brain using 17614-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 17614-1-AP (NDUFB2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of C2C12 using 17614-1-AP

  Immunofluorescent analysis of (4% PFA) fixed C2C12 cells using NDUFB2 antibody (17614-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HuH-7, cellules MCF-7, tissu cardiaque de rat, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de souris, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu cardiaque humain, tissu cérébral humain, tissu cutané humain, tissu hépatique humain, tissu ovarien humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

17614-1-AP cible NDUFB2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDUFB2 Protéine recombinante Ag11789
• Nom complet: NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 2, 8kDa
• Masse moléculaire calculée: 95 aa, 11 kDa
• Poids moléculaire observé: 8-12 kDa
• Numéro d’acquisition GenBank: BC063026
• Symbole du gène: NDUFB2
• Identification du gène (NCBI): 4708
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The multisubunit NADH:ubiquinone oxidoreductase (complex I) is the first enzyme complex in the electron transport chain of mitochondria. NDUFB2, also named as CI-AGGG, is a nuclear encoded subunit located in the hydrophobic protein fraction of complex I(PMID:9878551). It may have a role in the regulation of molecular changes during the global cardiac ischemia/reperfusion injury during cardiopulmonary bypass (CPB). The full length 12 kDa protein has a transit peptide with 33 amino acids.