Anticorps Polyclonal de lapin anti-GRIM19

• Phare
• Validé par KD/KO

GRIM19 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 10986-1-AP

Synonymes

B16.6, CDA016, CGI 39, CI B16.6, Complex I B16.6, GRIM 19, GRIM19, NDUFA13

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Galerie de données de validation

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  WB analysis using 10986-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 10986-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 10986-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10986-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10986-1-AP (GRIM19 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 10986-1-AP

  IP result of anti-GRIM19 (IP:10986-1-AP, 4ug; Detection:10986-1-AP 1:500) with HEK-293 cells lysate 2000ug.

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  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 10986-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 10986-1-AP (GRIM19 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, cellules MCF-7, tissu cardiaque de rat
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 4 publications below

Informations sur le produit

10986-1-AP cible GRIM19 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: GRIM19 Protéine recombinante Ag1435
• Nom complet: NADH dehydrogenase (ubiquinone) 1 alpha subcomplex, 13
• Masse moléculaire calculée: 16.5 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC009189
• Symbole du gène: GRIM19
• Identification du gène (NCBI): 51079
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GRIM19(Gene associated with retinoic and interferon-induced mortality 19 protein) is also named as NDUFA13 ,CI-B16.6 and belongs to the complex I NDUFA13 subunit family.t GRIM-19 may distribute in the mitochondria and/or nucleus depending on the cell type.Alternatively, its cellular localization may be regulated by post-translational modifications such as phosphorylation and acetylation. Lastly, GRIM-19 may exist in different isoforms that may be distributed in distinct cellular locations(PMID:12628925).

Publications

Species	Application	Title
human	WB	Dev Cell; PDGF signaling inhibits mitophagy in glioblastoma stem cells through N6-methyladenosine.; Authors - Deguan Lv
human	WB,IHC	Hum Gene Ther; Nonviral delivery of GRIM-19 gene inhibits tumor growth with reduced local and systemic complications.; Authors - Yang Xu
human	WB,IHC	Exp Cell Res; GRIM-19 inhibits proliferation and induces apoptosis in a p53-dependent manner in colorectal cancer cells through the SIRT7/PCAF/MDM2 axis.; Authors - Ding Wang ; KD Validated
human	WB,IHC	Cancer Chemother Pharmacol; PMID: 30032449; Authors - Feng Ni
		Int J Oncol; Atrazine promotes RM1 prostate cancer cell proliferation by activating STAT3 signaling.; Authors - Kebang Hu
human	WB,IHC	Oncol Lett; GRIM-19 deficiency promotes clear cell renal cell carcinoma progression and is associated with high TNM stage and Fuhrman grade.; Authors - Naimeng Yan