Anticorps Polyclonal de lapin anti-NDFIP1

NDFIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 15602-1-AP

Synonymes

PSEC0223, PSEC0192, NEDD4 WW domain-binding protein 5, NEDD4 family-interacting protein 1, N4WBP5

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Galerie de données de validation

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  WB analysis using 15602-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15602-1-AP (NDFIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15602-1-AP

  IP result of anti-NDFIP1 (IP:15602-1-AP, 3ug; Detection:15602-1-AP 1:300) with HEK-293 cells lysate 3200ug.

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  IF Staining of HEK-293 using 15602-1-AP

  Immunofluorescent analysis of HEK-293 cells using 15602-1-AP (NDFIP1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IF/ICC: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below
• IP: See 2 publications below

Informations sur le produit

15602-1-AP cible NDFIP1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NDFIP1 Protéine recombinante Ag7985
• Nom complet: Nedd4 family interacting protein 1
• Masse moléculaire calculée: 25 kDa
• Poids moléculaire observé: 25-30 kDa
• Numéro d’acquisition GenBank: BC004317
• Symbole du gène: NDFIP1
• Identification du gène (NCBI): 80762
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Ndfip1 is an adaptor protein for the Nedd4 family of ubiquitin ligases and has been found to be upregulated in neurons after brain injury, including head trauma, stroke and metal toxicity. Recent studies confirmed the role of Ndfip1 as a regulator of iron metabolism and pointed out that Ndfip1 was involved in iron homeostasis by regulating the degradation of iron importer divalent metal transporter 1. Ndfip1 is a transmembrane protein that is localized to the Golgi and post-Golgi vesicles such as endosomes. It is an adaptor protein that recruits E3 ligases to ubiquitinate target proteins. It is ubiquitously expressed and contains 2 PY motifs, which interact with several Nedd4 family E3s to degradate proteins.

Publications

Species	Application	Title
human	IP	Mol Cell; PKCλ/ι inhibition activates an ULK2-mediated interferon response to repress tumorigenesis.; Authors - Juan F Linares
human	WB,IF,IP	Cell Death Discov; WWP1 upregulation predicts poor prognosis and promotes tumor progression by regulating ubiquitination of NDFIP1 in intrahepatic cholangiocarcinoma.; Authors - Yongjian Li