Anticorps Polyclonal de lapin anti-Nicastrin

• Phare
• Validé par KD/KO

Nicastrin Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 14071-1-AP

Synonymes

APH2, KIAA0253, NCSTN, nicastrin

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Galerie de données de validation

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  WB analysis of mouse brain using 14071-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of C6 using 14071-1-AP

  C6 cells were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 14071-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 14071-1-AP (NCSTN antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IF Staining of C6 using 14071-1-AP

  Immunofluorescent analysis of C6 cells using 14071-1-AP (Nicastrin antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules C6, tissu cérébral humain
• Résultats positifs en IF/ICC: cellules C6

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below

Informations sur le produit

14071-1-AP cible Nicastrin dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Nicastrin Protéine recombinante Ag5137
• Nom complet: nicastrin
• Masse moléculaire calculée: 709 aa, 78.4 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC047621
• Symbole du gène: Nicastrin
• Identification du gène (NCBI): 23385
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicastrin (NCT) is a type I transmembrane glycoprotein, which forms high molecular weight gamma-secretase complexes with with presenilin 1 and presenilin 2 (PMID: 16096051). The gamma-secretase is an intramembrane protease cleaves many type I membrane proteins including amyloid precursor protein (APP) and Notch, generating peptide fragments that are important signaling components (PMID: 16096062). The proteolytical processing of APP results in the release of amyloid-βpeptide, a principal component of the plaques found in the brains of Alzheimer's disease patients (PMID: 12716934).

Publications

Species	Application	Title
mouse	WB	Gut Microbes; Gut-derived β-amyloid: Likely a centerpiece of the gut-brain axis contributing to Alzheimer's pathogenesis; Authors - Jinghua Jin
mouse	WB	J Neuroinflammation; Far infrared light irradiation enhances Aβ clearance via increased exocytotic microglial ATP and ameliorates cognitive deficit in Alzheimer's disease-like mice.; Authors - Qingyong Li
human	WB	Front Oncol; Stimulation of Let-7 Maturation by Metformin Improved the Response to Tyrosine Kinase Inhibitor Therapy in an m6A Dependent Manner.; Authors - Kai Li
mouse	WB	Mol Neurobiol; Memantine, a Noncompetitive N-Methyl-D-Aspartate Receptor Antagonist, Attenuates Cerebral Amyloid Angiopathy by Increasing Insulin-Degrading Enzyme Expression.; Authors - Yasuteru Inoue
human	WB,IHC	Cancer Cell Int; Effect of nicastrin on hepatocellular carcinoma proliferation and apoptosis through PI3K/AKT signalling pathway modulation.; Authors - Xicheng Wang ; KD Validated
mouse	WB	Neurosci Lett; Metformin attenuates vascular pathology by increasing expression of insulin-degrading enzyme in a mixed model of cerebral amyloid angiopathy and type 2 diabetes mellitus.; Authors - Yasuteru Inoue