Anticorps Polyclonal de lapin anti-NCOR1

NCOR1 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, chIP, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 20018-1-AP

Synonymes

hCIT529I10, hN CoR, KIAA1047, N CoR, N CoR1, NCOR1, Nuclear receptor corepressor 1, TRAC1

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Galerie de données de validation

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  WB analysis of HepG2 using 20018-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20018-1-AP (NCOR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 20018-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 20018-1-AP (NCOR1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human cervical cancer using 20018-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20018-1-AP (NCOR1 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human cervical cancer using 20018-1-AP

  Immunohistochemical analysis of paraffin-embedded human cervical cancer using 20018-1-AP (NCOR1 antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of rat brain using 20018-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 20018-1-AP (NCOR1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of rat brain using 20018-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain using 20018-1-AP (NCOR1 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HepG2, cellules K-562
• Résultats positifs en IHC: tissu de cancer du col de l'utérus humain, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IP: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

20018-1-AP cible NCOR1 dans les applications de WB, IP, IHC, chIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear receptor co-repressor 1
• Masse moléculaire calculée: 270 kDa
• Poids moléculaire observé: 120 kDa
• Numéro d’acquisition GenBank: NM_006311
• Symbole du gène: NCOR1
• Identification du gène (NCBI): 9611
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCOR1, also named as KIAA1047 and TRAC1, belongs to the N-CoR nuclear receptor corepressors family. NCO1 mediates transcriptional repression by certain nuclear receptors. It is part of a complex which promotes histone deacetylation and the formation of repressive chromatin structures which may impede the access of basal transcription factors. The antibody recognizes the 103 kDa isoform of NCOR1.

Publications

Species	Application	Title
mouse,human	WB	Nat Commun; Pharmacological inhibition of Lin28 promotes ketogenesis and restores lipid homeostasis in models of non-alcoholic fatty liver disease; Authors - Evangelia Lekka
	chIP	PLoS Biol; Transcriptional programming of lipid and amino acid metabolism by the skeletal muscle circadian clock.; Authors - Kenneth Allen Dyar
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song
human	WB	Theranostics; Palmitoylation of PKCδ by ZDHHC5 in hypothalamic microglia presents as a therapeutic target for fatty liver disease; Authors - Yan-Hang Wang
	WB	Cell; Osr2 functions as a biomechanical checkpoint to aggravate CD8+ T cell exhaustion in tumor; Authors - Jinjia Zhang
mouse,rat	WB,IP,IHC	Cell Mol Life Sci; Novel role of NCoR1 in impairing spatial memory through the mediation of a novel interacting protein DEC2; Authors - Kuang-Min Cheng ; KO Validated