Anticorps Polyclonal de lapin anti-SRC3/NCOA3

• Phare
• Validé par KD/KO

SRC3/NCOA3 Polyclonal Antibody for WB, IF, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IF, IHC, ChIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 20032-1-AP

Synonymes

ACTR, AIB 1, AIB1, CBP interacting protein, KAT13B, NCoA 3, NCOA3, nuclear receptor coactivator 3, pCIP, RAC 3, RAC3, SRC 3, SRC3, SRC3/NCOA3, TNRC14, TNRC16, TRAM 1, TRAM1

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Galerie de données de validation

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  WB analysis of HeLa using 20032-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20032-1-AP (NCOA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20032-1-AP

  Immunofluorescent analysis of HepG2 cells, using NCOA3 antibody 20032-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa,
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

20032-1-AP cible SRC3/NCOA3 dans les applications de WB, IF, IHC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: nuclear receptor coactivator 3
• Masse moléculaire calculée: 155 kDa
• Poids moléculaire observé: 160 kDa
• Numéro d’acquisition GenBank: NM_006534
• Symbole du gène: NCOA3
• Identification du gène (NCBI): 8202
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCOA3, also named as AIB1, SRC3 and TRAM1, belongs to the SRC/p160 nuclear receptor coactivator family. NCOA3 is a nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. NOCA3 plays a central role in creating a multisubunit coactivator complex, which probably acts via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). NCOA3 also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit. The antibody can recognizes all the isoforms of NCOA3.

Publications

Species	Application	Title
human,mouse	ChIP	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator; Authors - Xuan Zheng ; KD Validated
human	WB	J Biol Chem; A potential target for liver cancer management, lysophosphatidic acid receptor 6 (LPAR6), is transcriptionally up-regulated by the NCOA3 coactivator.; Authors - Xuan Zheng ; KD Validated
human	WB,IHC	Biomed Pharmacother; PMID: 32036219; Authors - Yu Chen ; KD Validated
human	IHC	Medicine (Baltimore); Pathological discrimination between luteinized thecoma associated with sclerosing peritonitis and thecoma; Authors - Jia Liu
human	IHC	Ann Diagn Pathol; Evaluation of the diagnostic utility of NCOA3, Maspin and VHL protein expression in pancreatic ductal adenocarcinoma: An immunohistochemical study; Authors - Noha Elkady