Anticorps Polyclonal de lapin anti-NCK2

• Phare
• Validé par KD/KO

NCK2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 10206-1-AP

Synonymes

Cytoplasmic protein NCK2, GRB4, Nck 2, NCK adaptor protein 2, NCK2, NCKbeta

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Galerie de données de validation

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  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of U-937 using 10206-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 10206-1-AP (NCK2 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human placenta using 10206-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 10206-1-AP (NCK2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules U-937,
• Résultats positifs en IHC: tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below

Informations sur le produit

10206-1-AP cible NCK2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCK2 Protéine recombinante Ag0262
• Nom complet: NCK adaptor protein 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC000103
• Symbole du gène: NCK2
• Identification du gène (NCBI): 8440
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Lots of the protein-protein interactions that are essential for eukaryotic intracellular signal transduction are mediated by protein binding modules including SH2, SH3, and LIM domains. NCK is a SH3- and SH2-containing adaptor protein implicated in coordinating various signaling pathways, including those of growth factor receptors and cell adhesion receptors. NCK-2 is a ubiquitously expressed and NCK-related adaptor protein comprising primarily three N-terminal SH3 domains and one C-terminal SH2 domain. NCK-2 interacts with PINCH, a LIM-only protein implicated in integrin-linked kinase signaling, and recognizes several key components of growth factor kinase-signaling pathways including EGF receptors, PDGF receptor, and IRS-1. NCK-2 may thus function as an adaptor protein connecting the growth factor receptor-signaling pathways with the integrin-signaling pathways. NCK-2 also interacts with focal adhesion kinase (FAK), a cytoplasmic protein tyrosine kinase critically involved in the cellular control of motility.

Publications

Species	Application	Title
mouse	WB	Evid Based Complement Alternat Med; Icariin Alleviates Glucocorticoid-Induced Osteoporosis through EphB4/Ephrin-B2 Axis.; Authors - Mi Huang
human	WB	Biochem Pharmacol; KLF4 suppresses anticancer effects of brusatol via transcriptional upregulating NCK2 expression in melanoma; Authors - Xiaodong Li ; KD Validated