Anticorps Polyclonal de lapin anti-NCAPH

• Phare
• Validé par KD/KO

NCAPH Polyclonal Antibody for WB, IHC, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, FC (Intra), IP, ELISA, IF

Conjugaison

Non conjugué

Publications

9

N° de cat : 11515-1-AP

Synonymes

CND2, Chromosome-associated protein H, CAPH, CAP H, BRRN1

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Galerie de données de validation

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  WB analysis using 11515-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11515-1-AP (NCAPH antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11515-1-AP

  WB result of NCAPH antibody (11515-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCAPH transfected HEK-293 cells.

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  IP experiment of HeLa using 11515-1-AP

  IP result of anti-NCAPH (IP:11515-1-AP, 3ug; Detection:11515-1-AP 1:800) with HeLa cells lysate 3000ug.

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  IHC staining of human colon cancer using 11515-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer using 11515-1-AP (NCAPH antibody) at dilution of 1:50 (under 10x lens).

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  FC experiment of HEK-293 using 11515-1-AP

  1x10^6 HEK-293 cells were intracellularly stained with 0.4 ug NCAPH Polyclonal antibody (11515-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.4 ug Rabbit IgG control Rabbit PolyAb (30000-0-AP) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en FC (Intra): cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 4 publications below
• IF: See 2 publications below

Informations sur le produit

11515-1-AP cible NCAPH dans les applications de WB, IHC, FC (Intra), IP, ELISA, IF et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAPH Protéine recombinante Ag2076
• Nom complet: non-SMC condensin I complex, subunit H
• Masse moléculaire calculée: 741 aa, 83 kDa
• Poids moléculaire observé: 83-100 kDa
• Numéro d’acquisition GenBank: BC024211
• Symbole du gène: NCAPH
• Identification du gène (NCBI): 23397
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Non-SMC condensin I complex subunit H (NCAPH) is one of the three non-SMC subunits in condensin I, which belongs to a recently defined superfamily of proteins termed kleisins. Another two non-SMC subunits, CAP-D2 and CAP-G, share a highly degenerate repeating motif known as HEAT repeat. Some studies show that each subunit is essential for viability and plays an important role in mitotic chromosome architecture and segregation. In recent years, researchers found that the high expression of NCAPH was associated with poor prognosis in patients with non-small cell lung cancer and prostate cancer. Downregulation of NCAPH inhibited the proliferation, migration, and invasion of several cancer cells significantly. Moreover, NCAPH was involved in the regulation of mature chromosome condensation and DNA damage. These data suggest that NCAPH may be a key carcinogen involved in the development and progression of human malignant tumors. ( PMID: 28300828, PMID: 33311486 )

Publications

Species	Application	Title
human	WB,IHC	Int J Mol Sci; OCT1 Is a Poor Prognostic Factor for Breast Cancer Patients and Promotes Cell Proliferation via Inducing NCAPH.; Authors - Takuya Ogura ; KD Validated
human	WB	Cell Death Dis; NCAPH plays important roles in human colon cancer.; Authors - Liang Yin ; KD Validated
human	WB,IHC	Cell Cycle; NCAPH promotes cell proliferation and inhibits cell apoptosis of bladder cancer cells through MEK/ERK signaling pathway.; Authors - Bo Li ; KD Validated
human	WB	J Cell Sci; Ki-67 and condensins support the integrity of mitotic chromosomes through distinct mechanisms.; Authors - Masatoshi Takagi
human	WB	Sci Rep; NCAPH is a prognostic biomarker and associated with immune infiltrates in lung adenocarcinoma.; Authors - Chao Li
human	WB,IHC	Mol Carcinog; Non-SMC condensin I complex subunit H enhances proliferation, migration, and invasion of hepatocellular carcinoma.; Authors - Chengjun Sun ; KD Validated