Anticorps Polyclonal de lapin anti-NCAM2

NCAM2 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 13850-1-AP

Synonymes

NCAM21, NCAM-2, NCAM 2, N CAM 2

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Galerie de données de validation

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  WB analysis using 13850-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13850-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13850-1-AP (NCAM2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13850-1-AP

  IP result of anti-NCAM2 (IP:13850-1-AP, 3ug; Detection:13850-1-AP 1:1000) with mouse brain tissue lysate 4000ug.

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 13850-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 13850-1-AP (NCAM2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat, tissu cérébral humain
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, tissu de gliome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

13850-1-AP cible NCAM2 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCAM2 Protéine recombinante Ag4821
• Nom complet: neural cell adhesion molecule 2
• Masse moléculaire calculée: 93 kDa
• Poids moléculaire observé: 125 kDa
• Numéro d’acquisition GenBank: BC052946
• Symbole du gène: NCAM2
• Identification du gène (NCBI): 4685
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The mammalian NCAM family has two members, NCAM1 and NCAM2 (PMID: 9729879; 20439718). Neural cell adhesion molecule 2 (NCAM2, also known as NCAM21) is a single-pass type I membrane protein that is highly expressed in the brain, which may play important roles in selective fasciculation and zone-to-zone projection of the primary olfactory axons or in neurodegenerative diseases such as Alzheimer's disease (PMID: 26611261). NCAM2 has two isoforms: NCAM2.1 bearing transmembrane and cytoplasmic domains and the shorter NCAM2.2 isoform, which is GPI-anchored (PMID: 9334170; 9221781). The molecular weight of NCAM2 is around 125 kDa and 93 kDa (PMID: 9334170; 3171685).

Publications

Species	Application	Title
mouse	IHC	Science; Breast cancer exploits neural signaling pathways for bone-to-meninges metastasis; Authors - Andrew E Whiteley
		J Comp Neurol; Illuminating the terminal nerve: Uncovering the link between GnRH-1 neuron and olfactory development; Authors - Enrico Amato
mouse	IHC	bioRxiv; Illuminating the Terminal Nerve: Uncovering the Link between GnRH-1 and Olfactory Development; Authors - Enrico Amato