Anticorps Polyclonal de lapin anti-NCALD

• Phare
• Validé par KD/KO

NCALD Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

10

N° de cat : 12925-1-AP

Synonymes

NCALD, neurocalcin delta, neurocalcin δ

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Galerie de données de validation

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  WB analysis of mouse brain using 12925-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 12925-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A549 using 12925-1-AP

  WB result of NCALD antibody (12925-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NCALD transfected A549 cells.

• 

  WB analysis of mouse cerebellum using 12925-1-AP

  mouse cerebellum tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 12925-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 12925-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human cerebellum using 12925-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 12925-1-AP (NCALD antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse cerebellum using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse cerebellum using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 12925-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12925-1-AP (NCALD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de souris, tissu de cervelet humain
• Résultats positifs en IHC: tissu de cervelet de souris, tissu cérébral de souris, tissu cérébral humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 8 publications below
• IHC: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

12925-1-AP cible NCALD dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NCALD Protéine recombinante Ag3614
• Nom complet: neurocalcin delta
• Masse moléculaire calculée: 193 aa, 22 kDa
• Poids moléculaire observé: 18-22 kDa
• Numéro d’acquisition GenBank: BC036098
• Symbole du gène: NCALD
• Identification du gène (NCBI): 83988
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NCALD belongs to the recoverin family. And it is a member of the EF-hand calcium-binding protein superfamily. NCALD may be involved in the calcium-dependent regulation of rhodopsin phosphorylation. It plays an important role in the regulation of the neuronal signal transduction process. NCALD is a likely candidate for conferring susceptibility to diabetic nephropathy.

Publications

Species	Application	Title
mouse	IHC	Genes Dev; Gsx2 controls region-specific activation of neural stem cells and injury-induced neurogenesis in the adult subventricular zone.; Authors - López-Juárez Alejandro A
mouse	WB	Am J Hum Genet; Neurocalcin Delta Suppression Protects against Spinal Muscular Atrophy in Humans and across Species by Restoring Impaired Endocytosis.; Authors - Markus Riessland ; KO Validated
human	WB,IHC	Oncotarget; Upregulation of long intergenic noncoding RNA 00673 promotes tumor proliferation via LSD1 interaction and repression of NCALD in non-small-cell lung cancer.; Authors - Xuefei Shi
human	WB,IHC	Int J Oncol; N6‑methyladenosine upregulates miR‑181d‑5p in exosomes derived from cancer‑associated fibroblasts to inhibit 5‑FU sensitivity by targeting NCALD in colorectal cancer.; Authors - Shengli Pan
human	WB	Front Neurol; Transcriptome and Proteome Analysis in LUHMES Cells Overexpressing Alpha-Synuclein.; Authors - Matthias Höllerhage
human	IHC,WB	Biomark Med; NCALD as a potential predictive biomarker for the efficacy of platinum-based chemotherapy in ovarian cancer; Authors - Sarah M Konrad