Anticorps Polyclonal de lapin anti-NARF

NARF Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 12173-1-AP

Synonymes

IOP2, NARF

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Galerie de données de validation

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  WB analysis of human liver using 12173-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 12173-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 12173-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human lung using 12173-1-AP

  human lung tissue were subjected to SDS PAGE followed by western blot with 12173-1-AP (NARF antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu hépatique humain, tissu placentaire humain, tissu pulmonaire humain, tissu testiculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

12173-1-AP cible NARF dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NARF Protéine recombinante Ag2816
• Nom complet: nuclear prelamin A recognition factor
• Masse moléculaire calculée: 46 kDa, 53 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC016440
• Symbole du gène: NARF
• Identification du gène (NCBI): 26502
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Prelamin A, is processed in the nucleus to lamin A by removal of its final 18 amino acids, including the cysteine residue in its C-terminal CAAX box, which is farnesylated. Nuclear prelamin A recognition factor (NARF) binds the farnesylated prelamin A C-terminal domain(preAct)(PMID:10514485). NARF, at 52 kDa, also named as IOP2, is a small enough protein to freely diffuse through the nuclear pore complex and may be retained within the nucleus by binding nuclear proteins and it has all of the information necessary to be imported into the nucleus(PMID:10514485). It has 3 isoforms produced by alternative splicing with the molecular mass of 51 kDa, 56 kDa and 46 kDa,respectively.