Anticorps Polyclonal de lapin anti-NAPRT1

NAPRT1 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

16

N° de cat : 13549-1-AP

Synonymes

FHA HIT interacting protein, FHIP, NAPRT1, NAPRTase, PP3856

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Galerie de données de validation

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  WB analysis using 13549-1-AP

  Mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human colon using 13549-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 13549-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 13549-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human small intestine using 13549-1-AP

  human small intestine tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 13549-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 13549-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 13549-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 13549-1-AP (NAPRT1 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HEK-293 using 13549-1-AP

  IP result of anti-NAPRT1 (IP:13549-1-AP, 4ug; Detection:13549-1-AP 1:1000) with HEK-293 cells lysate 2800ug.

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, cellules HEK-293, cellules HepG2, cellules L02, tissu de côlon humain, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 15 publications below
• IHC: See 4 publications below
• IF: See 3 publications below

Informations sur le produit

13549-1-AP cible NAPRT1 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAPRT1 Protéine recombinante Ag4265
• Nom complet: nicotinate phosphoribosyltransferase domain containing 1
• Masse moléculaire calculée: 514 aa, 55 kDa
• Poids moléculaire observé: 51 kDa, 56 kDa
• Numéro d’acquisition GenBank: BC032466
• Symbole du gène: NAPRT1
• Identification du gène (NCBI): 93100
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Nicotinic acid (NA) is a coenzyme in cellular redox reactions, and is an essential component of metabolic pathways in all living cells. NAPRT1 (Nicotinate phosphoribosyltransferase) is essential for increasing cellular NAD levels and, thus, to prevent oxidative stress of cells. NAPRT1 converts Nicotinic acid (NA; niacin) to NA mononucleotide (NaMN), which is then converted to NA adenine dinucleotide (NaAD), and finally to nicotinamide adenine dinucleotide (NAD).

Publications

Species	Application	Title
human,mouse	WB	Cell Metab; NAD+ Metabolism Maintains Inducible PD-L1 Expression to Drive Tumor Immune Evasion.; Authors - Hongwei Lv
human	IF,WB	J Pharm Anal; Epigenetic drug library screening reveals targeting DOT1L abrogates NAD+ synthesis by reprogramming H3K79 methylation in uveal melanoma; Authors - Xiang Gu
	WB	Kidney Int; Anticystogenic activity of a small molecule PAK4 inhibitor may be a novel treatment for autosomal dominant polycystic kidney disease.; Authors - Vicki J Hwang
human	WB	Clin Cancer Res; Targeting of NAD metabolism in pancreatic cancer cells: potential novel therapy for pancreatic tumors.; Authors - Chini Claudia Cs CC
human,mouse	WB,IHC	Int J Mol Sci; NAD+ Anabolism Disturbance Causes Glomerular Mesangial Cell Injury in Diabetic Nephropathy.; Authors - Xue Li
human,mouse	IF	Metabolism; Hyperglycemia-reduced NAD+ biosynthesis impairs corneal epithelial wound healing in diabetic mice.; Authors - Ya Li