Anticorps Polyclonal de lapin anti-NAP1L4

• Phare
• Validé par KD/KO

NAP1L4 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 16018-1-AP

Synonymes

hNAP2, NAP 2, NAP1L4, NAP2, NAP2L, Nucleosome assembly protein 2

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Galerie de données de validation

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  WB analysis of HeLa using 16018-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 16018-1-AP

  WB result of NAP1L4 antibody (16018-1-AP; 1:6000; incubated at room temperature for 1.5 hours) with sh-Control and sh-NAP1L4 transfected HeLa cells.

• 

  WB analysis of mouse liver using 16018-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 16018-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16018-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 16018-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 16018-1-AP (NAP1L4 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16018-1-AP

  IP result of anti-NAP1L4 (IP:16018-1-AP, 4ug; Detection:16018-1-AP 1:1000) with HeLa cells lysate 4000ug.

• 

  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human liver using 16018-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 16018-1-AP (NAP1L4 antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A431, cellules HEK-293, tissu hépatique de souris, tissu testiculaire de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

16018-1-AP cible NAP1L4 dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NAP1L4 Protéine recombinante Ag8827
• Nom complet: nucleosome assembly protein 1-like 4
• Masse moléculaire calculée: 375 aa, 43 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC022090
• Symbole du gène: NAP1L4
• Identification du gène (NCBI): 4676
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	J Cell Biol; Phase separation and toxicity of C9orf72 poly(PR) depends on alternate distribution of arginine.; Authors - Chen Chen
human	WB	Mol Cell Proteomics; A novel immuno-competitive capture mass spectrometry strategy for protein-protein interaction profiling reveals that LATS kinases regulate HCV replication through NS5A phosphorylation.; Authors - Hélène Meistermann
mouse	IF,WB	J Virol; NAP1L1 and NAP1L4 binding to Hypervariable Domain of Chikungunya Virus nsP3 Protein is bivalent and requires phosphorylation.; Authors - Francisco Dominguez
human	WB,IHC	J Dermatol Sci; Nucleosome assembly protein 1-like 4, a new therapeutic target for proliferation and invasion of melanoma cells.; Authors - Satoru Mizuhashi ; KD Validated
human	WB	Nat Struct Mol Biol; Mechanism of client selection by the protein quality-control factor UBE2O; Authors - Matthew C J Yip
human	WB	iScience; Differential toxicity and localization of arginine-rich C9ORF72 dipeptide repeat proteins depend on de-clustering of positive charges; Authors - Tamami Miyagi