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Anticorps Recombinant de lapin anti-NADK

NADK Recombinant Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242502B7

N° de cat : 84996-1-PBS

Synonymes

242502B7, EC:2.7.1.23, NAD kinase, Poly(P)/ATP NAD kinase

Galerie de données de validation

Filter:
  • Western Blot (WB) analysis of various lysates using NADK Recombinant antibody (84996-1-RR)

    WB analysis using 84996-1-RR (same clone as 84996-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 84996-1-RR (NADK antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84996-1-PBS in a different storage buffer formulation.

  • Affinity and Kinetic Characterization of 84996-1-RR

    Affinity and Kinetic Characterization of 84996-1-RR

    Biolayer interferometry (BLl) kinetic assays of 84996-1-RR against Human NADK were performed. The affinity constant is 2.25 nM.

Informations sur le produit

84996-1-PBS cible NADK dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Hôte / Isotype Lapin / IgG
Clonalité Recombinant
Type Anticorps
Immunogène NADK Protéine recombinante Ag7906
Nom complet NAD kinase
Masse moléculaire calculée 49 kDa
Poids moléculaire observé 49-52 kDa
Numéro d’acquisition GenBankBC001709
Symbole du gène NADK
Identification du gène (NCBI) 65220
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

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