Anticorps Polyclonal de lapin anti-NADK

• Phare
• Validé par KD/KO

NADK Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, CoIP, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 15548-1-AP

Synonymes

NAD kinase, NADK, Poly(P)/ATP NAD kinase

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 15548-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of RAW264.7 using 15548-1-AP

  RAW264.7 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 15548-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 15548-1-AP (NADK antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 15548-1-AP (NADK antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human brain using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human brain using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human lung using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary using 15548-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 15548-1-AP (NADK antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15548-1-AP

  Immunofluorescent analysis of Hela cells, using NADK antibody 15548-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HeLa, RAW264.7
• Résultats positifs en IHC: tissu rénal humain, tissu cérébral humain, tissu ovarien humain, tissu pulmonaire humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15548-1-AP cible NADK dans les applications de WB, IHC, IF/ICC, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: NADK Protéine recombinante Ag7906
• Nom complet: NAD kinase
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 49 kDa
• Numéro d’acquisition GenBank: BC001709
• Symbole du gène: NADK
• Identification du gène (NCBI): 65220
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

NAD kinase (NADK) is the sole NADP+-biosynthetic enzyme that catalyzes phosphorylation of NAD+ to yield NADP+ using ATP as a phosphoryl donor, and thus, plays a vital role in the cell and represents a potentially powerful antimicrobial drug target (PMID:21526340). It belongs to the NAD kinase family. NADK has 3 isoforms with the molecular mass of 49, 63 and 46 kDa. The catalytically active human NADK is a homotetramer (PMID:11594753).

Publications

Species	Application	Title
mouse,human	IF	Cell Metab; Upregulation of Antioxidant Capacity and Nucleotide Precursor Availability Suffices for Oncogenic Transformation.; Authors - Yang Zhang ; KD Validated
human	IHC	Sci Adv; G6PD-mediated increase in de novo NADP+ biosynthesis promotes antioxidant defense and tumor metastasis.; Authors - Yang Zhang
	WB	Nucleic Acids Res; Human DNA ligase IV is able to use NAD+ as an alternative adenylation donor for DNA ends ligation.; Authors - Shih-Hsun Chen
human	IF	Nat Commun; NADP+ is an endogenous PARP inhibitor in DNA damage response and tumor suppression.; Authors - Chunjing Bian
human	WB	J Biol Chem; Glucose-6-phosphate dehydrogenase exerts anti-stress effects independently of its enzymatic activity; Authors - Xiaohan Jin
human	IF	Redox Biol; NADK-mediated de novo NADP(H) synthesis is a metabolic adaptation essential for breast cancer metastasis; Authors - Didem Ilter