Anticorps Monoclonal anti-MYH2-specific

MYH2-specific Monoclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1F1B6

N° de cat : 66212-1-Ig

Synonymes

MYH2, MYH2A, MYH2-specific, MYHas8, MyHC 2A, MyHC IIa, MYHSA2, Myosin 2, Myosin 2a, Myosin heavy chain 2, Myosin heavy chain 2a, Myosin heavy chain IIa

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Galerie de données de validation

• 

  WB analysis of human skeletal muscle using 66212-1-Ig

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 66212-1-Ig (Myosin 2a antibody) at dilution of 1:100000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse heart using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:4000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse tongue using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of mouse tongue using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse tongue tissue slide using 66212-1-Ig (MYH2-specific antibody) at dilution of 1:2000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 66212-1-Ig

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 66212-1-Ig (Myosin 2a Antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique humain,
• Résultats positifs en IHC: tissu cardiaque de souris, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain, tissu lingual de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:100000
• Immunohistochimie (IHC): IHC : 1:2000-1:8000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

66212-1-Ig cible MYH2-specific dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 2, skeletal muscle, adult
• Masse moléculaire calculée: 223 kDa
• Poids moléculaire observé: 200 kDa
• Numéro d’acquisition GenBank: NM_017534
• Symbole du gène: MYH2
• Identification du gène (NCBI): 4620
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH2 (Myosin II) is a member of the class II or conventional myosin heavy chains. Myosin II was first isolated from muscle but is also found in non muscle cells, and it is especially enriched in highly motile cell types such as amoebae. It forms bipolar filaments that interact with actin filaments to produce contraction. And it is the motor protein that generates force to drive muscle contraction. Functions in skeletal muscle contraction. The antibody is specific to MYH2, will not bind other myosins. It is a skeletal muscle specific antibody.

Publications

Species	Application	Title
	WB	J Agric Food Chem; Spermidine-Activated Satellite Cells Are Associated with Hypoacetylation in ACVR2B and Smad3 Binding to Myogenic Genes in Mice.; Authors - Luchu Zhang
	WB	Sci Rep; Trimetazidine and exercise provide comparable improvements to high fat diet-induced muscle dysfunction through enhancement of mitochondrial quality control.; Authors - Wenliang Zhang