Anticorps Monoclonal anti-IgG2b isotype control

IgG2b isotype control Monoclonal Antibody for FC

Hôte / Isotype

Mouse / IgG2b

Réactivité testée

Megathura crenulata

Applications

FC

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

11B8C4

N° de cat : CL488-66360-3

Synonymes

Mouse IgG2b, Mouse IgG2b isotype control

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Galerie de données de validation

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  FC experiment of HeLa using CL488-66360-3

  1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite® Plus 488 Mouse IgG2b isotype control (CL488-66360-3, Clone:11B8C4) (red), or 0.4 ug CoraLite® Plus 488-conjugated Beta Actin Monoclonal antibody (green) or unstained (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

Résultats positifs en cytométrie

Dilution recommandée

Application	Dilution
This reagent has been tested for flow cytometric analysis. It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-66360-3 cible IgG2b isotype control dans les applications de FC et montre une réactivité avec des échantillons Megathura crenulata

• Réactivité: Megathura crenulata
• Hôte / Isotype: Mouse / IgG2b
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: IgG2b isotype control
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.1% sodium azide and 0.5% BSA.
• Conditions de stockage: Store at 2-8°C. Avoid exposure to light. Stable for one year after shipment.

Informations générales

This antibody is used as the isotype control of mouse IgG2b. It can be used as the isotype control in Flow Cytometry and other experiments. The immunogen of this antibody is Keyhole Limpet Hemocyanin (KLH).

0.05-0.2 ug per test is suggested for FC experiment.