Anticorps Polyclonal de lapin anti-Marcks

• Phare
• Validé par KD/KO

Marcks Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

souris et plus (2)

Applications

WB, IP, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

12

N° de cat : 10004-2-Ig

Synonymes

Macs, Marcks, PKCSL

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Galerie de données de validation

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  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 10004-2-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 10004-2-Ig (Marcks antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse pancreas using 10004-2-Ig

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 10004-2-Ig (Marcks Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris,
• Résultats positifs en IHC: tissu pancréatique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IHC: See 2 publications below
• IF: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

10004-2-Ig cible Marcks dans les applications de WB, IP, IF, IHC, ELISA et montre une réactivité avec des échantillons souris

• Réactivité: souris
• Réactivité citée: rat, bovin, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Protéine recombinante
• Nom complet: myristoylated alanine rich protein kinase C substrate
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 88 kDa
• Numéro d’acquisition GenBank: BC046601
• Symbole du gène: Marcks
• Identification du gène (NCBI): 17118
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MARCKS is a member of MARCKS family of PKC substrate and it is also a widely accepted indicator of PKC activation. It shows a ubiquitous tissue distribution. The calculated molecular weight of MARCKS is 39 kDa and its apparent molecular weights were found at 68 kDa to 90 kDa in different species. This protein is a known to regulated cytoskeleton remodeling and membrane recycling. The anti-MARCKS antibody is raised against purified MARCKS protein from mouse brain using a method similar to that previously reported for MacMARCKS

Publications

Species	Application	Title
bovine	WB	Proc Natl Acad Sci U S A; MARCKS protein mediates hydrogen peroxide regulation of endothelial permeability.; Authors - Jin Benjamin Y BY
mouse	IF	J Cell Sci; Dynamic adhesions and MARCKS in melanoma cells.; Authors - Estrada-Bernal Adriana A
	WB,IP	Carcinogenesis; GRB-7 facilitates HER-2/Neu-mediated signal transduction and tumor formation.; Authors - Bai Tao T
	WB,IHC	J Biol Chem; The long noncoding RNA lncZic2 drives the self-renewal of liver tumor-initiating cells via the protein kinase C substrates MARCKS and MARCKSL1.; Authors - Zhenzhen Chen ; KO Validated
mouse	WB,IHC	Endocrinology; Accelerated proliferation and differential global gene expression in pancreatic islets of five-week-old heterozygous Men1 mice: Men1 is a haploinsufficient suppressor.; Authors - Lejonklou Margareta H MH
	WB,IF	Mol Biol Cell; Myristoylated, alanine-rich C-kinase substrate phosphorylation regulates growth cone adhesion and pathfinding.; Authors - Gatlin Jesse C JC