Anticorps Polyclonal de lapin anti-MYST2

• Phare
• Validé par KD/KO

MYST2 Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 13751-1-AP

Synonymes

KAT7, KAT 7, Histone acetyltransferase KAT7, HBOa, HBO1

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Galerie de données de validation

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  WB analysis of Jurkat using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 13751-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 13751-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 13751-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 13751-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 13751-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 13751-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13751-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13751-1-AP (MYST2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 13751-1-AP

  IP result of anti-MYST2 (IP:13751-1-AP, 4ug; Detection:13751-1-AP 1:500) with HeLa cells lysate 2480ug.

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  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse kidney using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 13751-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer using 13751-1-AP (MYST2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of NIH/3T3 using 13751-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed NIH/3T3 cells using 13751-1-AP (MYST2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat, cellules MCF-7, cellules NIH/3T3, cellules PC-3, tissu testiculaire humain
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de tumeur ovarienne humain, tissu de cancer du sein humain, tissu rénal de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules NIH/3T3

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 15 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13751-1-AP cible MYST2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYST2 Protéine recombinante Ag4693
• Nom complet: MYST histone acetyltransferase 2
• Masse moléculaire calculée: 611 aa, 71 kDa
• Poids moléculaire observé: 83 kDa
• Numéro d’acquisition GenBank: BC032640
• Symbole du gène: MYST2
• Identification du gène (NCBI): 11143
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYST2, also named as HBO1, HBOa MOZ, YBF2/SAS3, SAS2 and TIP60 protein 2, belongs to the MYST (SAS/MOZ) family. It specifically represses AR mediated transcription. MYST2 is a candidate oncogene. It enhances the anchorage-independent growth of breast cancer cells.(PMID:19372580) MYST2 is a histone acetyltransferase (HAT) which could exert oncogenic function in breast cancer. It is an important downstream molecule of ERa, and ERK1/2 signaling pathway may involved in the expression of HBO1 increased by E2.

Publications

Species	Application	Title
human	WB	Cancer Cell; Transcriptional Regulation of the Warburg Effect in Cancer by SIX1.; Authors - Ling Li ; KO Validated
human	WB,IF,IP	Autophagy; KAT7-mediated CANX (calnexin) crotonylation regulates leucine-stimulated MTORC1 activity.; Authors - Guokai Yan
human	WB	Cancer Res; Hbo1 is a cyclin E/CDK2 substrate that enriches breast cancer stem-like cells.; Authors - Mylinh T Duong
human	WB	Mol Cell Proteomics; Acetylproteomic analysis reveals functional implications of lysine acetylation in human spermatozoa (sperm).; Authors - Heguo Yu
human	WB	Mol Cell Proteomics; Regulation of acetylation restores proteolytic function of diseased myocardium in mouse and human.; Authors - Ding Wang
human	WB	Mol Cell Biol; HBO1 (KAT7) Does Not Have an Essential Role in Cell Proliferation, DNA Replication, or Histone 4 Acetylation in Human Cells.; Authors - Andrew J Kueh