Anticorps Polyclonal de lapin anti-MYSM1-Specific

• Phare
• Validé par KD/KO

MYSM1-Specific Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 20078-1-AP

Synonymes

2A DUB, 2ADUB, KIAA1915, MYSM1, MYSM1-Specific, RP4 592A1.1

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Galerie de données de validation

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  WB analysis of A549 using 20078-1-AP

  WB result of MYSM1-Specific antibody (20078-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYSM1-Specific transfected A549 cells.

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  WB analysis of HeLa using 20078-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 20078-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 20078-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 20078-1-AP

  A549 cells were subjected to SDS PAGE followed by western blot with 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 20078-1-AP

  IP result of anti-MYSM1-Specific (IP:20078-1-AP, 4ug; Detection:20078-1-AP 1:300) with HeLa cells lysate 3200ug.

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  IHC staining of mouse small intestine using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human pancreas cancer using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human urothelial carcinoma using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human urothelial carcinoma tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat kidney using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat small intestine using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded rat small intestine tissue slide using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human spleen using 20078-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of HeLa using 20078-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed HeLa cells using 20078-1-AP (MYSM1-Specific antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HEK-293, cellules HeLa, cellules HepG2
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal de souris, tissu de cancer du pancréas humain, tissu de carcinome urothélial humain, tissu d'intestin grêle de rat, tissu d'intestin grêle de souris, tissu rénal de rat, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

20078-1-AP cible MYSM1-Specific dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: Myb-like, SWIRM and MPN domains 1
• Masse moléculaire calculée: 95 kDa
• Poids moléculaire observé: 81 kDa
• Numéro d’acquisition GenBank: NM_001085487
• Symbole du gène: MYSM1
• Identification du gène (NCBI): 114803
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYSM1, also named as KIAA1915, belongs to the peptidase M67A family and MYSM1 subfamily. MYSM1 is a metalloprotease that specifically deubiquitinates monoubiquitinated histone H2A, a specific tag for epigenetic transcriptional repression, thereby acting as a coactivator. Deubiquitination of histone H2A leads to facilitate the phosphorylation and dissociation of histone H1 from the nucleosome. MYSM1 acts as a coactivator by participating in the initiation and elongation steps of androgen receptor (AR)-induced gene activation. It catalyzes the reaction: Ubiquitin C-terminal thioester + H2O = ubiquitin + a thiol. MYSM1 is a marker of cancer. The antibody is specific to MYSM1.

Publications

Species	Application	Title
human	IHC	Cell Death Discov; MYSM1 induces apoptosis and sensitizes TNBC cells to cisplatin via RSK3-phospho-BAD pathway.; Authors - Xiaolin Guan