Anticorps Polyclonal de lapin anti-MYPN-Specific

MYPN-Specific Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 16180-1-AP

Synonymes

145 kDa sarcomeric protein, MYOP, myopalladin, MYPN

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Galerie de données de validation

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  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16180-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16180-1-AP (MYPN-Specific antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human skeletal muscle using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16180-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16180-1-AP (MYPN-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16180-1-AP

  Immunofluorescent analysis of HepG2 cells using 16180-1-AP (MYPN-Specific antibody) at dilution of 1:25 and Rhodamine-Goat anti-Rabbit IgG.

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Applications testées

• Résultats positifs en WB: cellules HeLa
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

16180-1-AP cible MYPN-Specific dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myopalladin
• Masse moléculaire calculée: 145 kDa
• Poids moléculaire observé: 145 kDa
• Numéro d’acquisition GenBank: NM_032578
• Symbole du gène: MYPN
• Identification du gène (NCBI): 84665
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYPN is a component of the sarcomere that tethers nebulin in skeletal muscle and nebulette in cardiac muscle to alpha-actinin at the Z lines (from OMIM). Myopalladin and palladin share C-terminal fragment in sequence. While myopalladin are most abundant in skeletal and cardiac muscle, palladin is ubiquitously expressed in the organs of developing vertebrates. This antibody is specific to myopalladin.

Publications

Species	Application	Title
human	WB	Sci Rep; Label-free Quantitative Analysis of Protein Expression Alterations in miR-26a-Knockout HeLa Cells using SWATH-MS Technology.; Authors - Hexiao Shen