Anticorps Polyclonal de lapin anti-MYOZ3

MYOZ3 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 23880-1-AP

Synonymes

Calsarcin 3, CS 3, CS3, FATZ related protein 3, FRP3, MYOZ3, myozenin 3

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Galerie de données de validation

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  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skeletal muscle using 23880-1-AP

  rat skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 23880-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 23880-1-AP (MYOZ3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human skeletal muscle using 23880-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 23880-1-AP (MYOZ3 Antibody) at dilution of 1:50 (under 10x lens).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

23880-1-AP cible MYOZ3 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOZ3 Protéine recombinante Ag20839
• Nom complet: myozenin 3
• Masse moléculaire calculée: 251 aa, 27 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC074870
• Symbole du gène: MYOZ3
• Identification du gène (NCBI): 91977
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Martina (Verified Customer) (07-03-2023)

IF protocol for fixed cryosection of mouse skeletal muscle: after a quick permeabilization step with Triton 0,3% in PBS, block with BSA 0,5% and GS 10% in PBS for at least 2 hours at room temperature. Then incubate with primary antibody 1:100 in blocking solution overnight at +4°C. The day after, incubate with secondary antibody 1:200 in blocking solution for 2 hours at room temperature. WB protocol for mouse skeletal muscle proteins: block the membrane with 5% milk in TBS-T for at least 2 hours at room temperature; then incubate with primary antibody 1:1000 in 5% milk in TBS-T at +4°C overnight. The day after, incubate with secondary antibody 1:2000 in 5% milk in TBS-T for 2 hours at room temperature. Developing the membrane, you will see double bands between 39 and 28 kDa.

• Applications: Western Blot, Immunofluorescence
• Cell Tissue Type: Murine skeletal muscle

MYOZ3 Antibody Western Blot,Immunofluorescence validation ( dilution) in Murine skeletal muscle (Cat no:23880-1-AP)