Anticorps Polyclonal de lapin anti-MYOT

MYOT Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, IHC, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 10731-1-AP

Synonymes

57 kDa cytoskeletal protein, LGMD1, LGMD1A, MYOT, myotilin, TTID

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Galerie de données de validation

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  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10731-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 10731-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10731-1-AP (MYOT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 10731-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10731-1-AP (MYOT antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de souris, tissu cardiaque de souris
• Résultats positifs en IHC: tissu de muscle squelettique de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

10731-1-AP cible MYOT dans les applications de WB, IF, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYOT Protéine recombinante Ag1112
• Nom complet: myotilin
• Masse moléculaire calculée: 55 kDa
• Poids moléculaire observé: 55-57 kDa, 35 kDa
• Numéro d’acquisition GenBank: BC005376
• Symbole du gène: MYOT
• Identification du gène (NCBI): 9499
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOT (myotilin) is a structural protein of the striated muscle Z-discs. It interacts with both actinin and filamin, forming a complex to maintain structural stability of muscles. In adult tissues, myotilin is mainly expressed in skeletal and cardiac muscles and in the peripheral nerves. Missense mutations of myotilin cause limb girdle muscular dystrophy 1A and some other myopathy. Two isoforms of MYOT exist due to the alternative splicing. This antibody can detect both of isoforms around 57 kDa and 35 kDa.

Publications

Species	Application	Title
human	IHC	Neurology; Mutations in HSPB8 causing a new phenotype of distal myopathy and motor neuropathy.; Authors - Roula Ghaoui
human	WB	Am J Pathol; In vivo characterization of mutant myotilins.; Authors - Keduka Etsuko E
		Neurol Genet; Novel mutation in TNPO3 causes congenital limb-girdle myopathy with slow progression.; Authors - Anna Vihola
mouse	IHC	Elife; Isoform-specific mutation in Dystonin-b gene causes late-onset protein aggregate myopathy and cardiomyopathy; Authors - Nozomu Yoshioka
human	IF	Eur J Neurol; Novel mutations in DNAJB6 cause LGMD1D and distal myopathy in French families; Authors - P H Jonson
human	IHC	Neurol Neuroimmunol Neuroinflamm; Clinicopathologic Profiles of Sporadic Late-Onset Nemaline Myopathy: Practical Importance of Anti-α-Actinin Immunostaining; Authors - Bing Zhao