Anticorps Polyclonal de lapin anti-MYOM1-Specific

MYOM1-Specific Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, RIP, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 20360-1-AP

Synonymes

MYOM1, Myomesin-1, Myomesin family member 1, Myomesin 1, 190 kDa titin-associated protein

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Galerie de données de validation

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  WB analysis using 20360-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 20360-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skeletal muscle using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 20360-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 20360-1-AP (MYOM1-Specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse skeletal muscle using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse skeletal muscle tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse heart using 20360-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 20360-1-AP (MYOM1-Specific antibody) at dilution of 1:100 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 20360-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYOM1-Specific antibody (20360-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de muscle squelettique humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de muscle squelettique de souris, tissu cardiaque de souris
• Résultats positifs en IF/ICC: cellules C2C12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• RIP: See 1 publications below

Informations sur le produit

20360-1-AP cible MYOM1-Specific dans les applications de WB, IHC, IF/ICC, IF-P, RIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myomesin 1, 185kDa
• Masse moléculaire calculée: 188 kDa
• Poids moléculaire observé: 190 kDa
• Numéro d’acquisition GenBank: NM_003803
• Symbole du gène: MYOM1
• Identification du gène (NCBI): 8736
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYOM1 is a major component of the vertebrate myofibrillar M band. MYOM1 binds myosin, titin, and light meromyosin. This binding is dose-dependent. The antibody is specific to MYOM1.

Publications

Species	Application	Title
mouse	WB	Sci Rep; Hypertension reduces soluble guanylyl cyclase expression in the mouse aorta via the Notch signaling pathway.; Authors - Catarina Rippe
mouse	WB	J Cell Mol Med; Hydrogen sulphide ameliorating skeletal muscle atrophy in db/db mice via Muscle RING finger 1 S-sulfhydration.; Authors - Fangping Lu
mouse	WB,RIP	iScience; Splicing factor Srsf5 deletion disrupts alternative splicing and causes noncompaction of ventricular myocardium; Authors - Xiaoli Zhang, Ze
rat	IHC,IF	Cell Signal; Long non-coding RNA Pvt1 modulates the pathological cardiac hypertrophy via miR-196b-mediated OSMR regulation.; Authors - Qingqing Wu
rat	IF	Int J Mol Sci; Skeletal Muscle Dysfunction in Experimental Pulmonary Hypertension; Authors - Kosmas Kosmas