Anticorps Polyclonal de lapin anti-Myocilin

Myocilin Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 14238-1-AP

Synonymes

GLC1A, GPOA, JOAG, JOAG1, MYOC, Myocilin, TIGR

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Galerie de données de validation

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  WB analysis of human heart using 14238-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse skeletal muscle using 14238-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human stomach using 14238-1-AP

  human stomach tissue were subjected to SDS PAGE followed by western blot with 14238-1-AP (Myocilin antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse skeletal muscle using 14238-1-AP

  IP result of anti-Myocilin (IP:14238-1-AP, 4ug; Detection:14238-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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Applications testées

• Résultats positifs en WB: tissu cardiaque humain, tissu de muscle squelettique de souris, tissu d'estomac humain
• Résultats positifs en IP: tissu de muscle squelettique de souris

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

14238-1-AP cible Myocilin dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Myocilin Protéine recombinante Ag5492
• Nom complet: myocilin, trabecular meshwork inducible glucocorticoid response
• Masse moléculaire calculée: 57 kDa
• Poids moléculaire observé: 66 kDa
• Numéro d’acquisition GenBank: BC029261
• Symbole du gène: Myocilin
• Identification du gène (NCBI): 4653
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myocilin (MYOC), also known as TIGR or GLC1A, is a secreted glycoprotein of the olfactomedin family, originally identified in trabecular meshwork cells after prolonged treatment with glucocorticoids, and, independently, in the retina (PMID: 9176893; 9169133). It may participate in the obstruction of fluid outflow in the trabecular meshwork. Defects in MYOC are the cause of primary open angle glaucoma (POAG) (PMID: 21709622). MYOC has a molecular weight of 55-57 kDa, besides, a 66-kDa form, which could be a result of post-translational modifications, has also been reported in some researches (PMID: 9497363; 12150989).

Publications

Species	Application	Title
human	WB	Ann Transl Med; Adenosine A3 receptor activated in H2O2 oxidative stress of primary open-angle glaucoma.; Authors - Ziyu Zhou
human	WB	Int J Ophthalmol; Expression profile analysis to identify potential gene changes induced by dexamethasone in the trabecular meshwork; Authors - Miao Wei
mouse	WB	J Ocul Pharmacol Ther; Activation of Sonic Hedgehog Signaling Pathway Regulates Human Trabecular Meshwork Cell Function; Authors - Xiaochen Wang