Anticorps Polyclonal de lapin anti-MYL3

MYL3 Polyclonal Antibody for WB, IP, IF, IHC, ELISA, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 10913-1-AP

Synonymes

Cardiac myosin light chain 1, CMH8, CMLC1, MLC1SB, MLC1V, MYL3, Myosin light chain 3, VLC1

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Galerie de données de validation

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  WB analysis of mouse heart using 10913-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 10913-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:30000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 10913-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 10913-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 10913-1-AP (MYL3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 10913-1-AP

  IP result of anti-MYL3 (IP:10913-1-AP, 3ug; Detection:10913-1-AP 1:7000) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 10913-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 10913-1-AP (MYL3 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of C2C12 using 10913-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using MYL3 antibody (10913-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  FC experiment of C2C12 using 10913-1-AP

  1X10^6 C2C12 cells were intracellularly stained with 0.4 ug Anti-Human MYL3 (10913-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat, tissu cardiaque humain, tissu de muscle squelettique de souris
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules C2C12,
• Résultats positifs en FC (Intra): cellules C2C12
• Résultats positifs en cytométrie: cellules C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension
• Flow Cytometry (FC): FC : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

10913-1-AP cible MYL3 dans les applications de WB, IHC, IF/ICC, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL3 Protéine recombinante Ag1364
• Nom complet: myosin, light chain 3, alkali; ventricular, skeletal, slow
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22-27 kDa
• Numéro d’acquisition GenBank: BC009790
• Symbole du gène: MYL3
• Identification du gène (NCBI): 4634
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYL3, also named as MLC1v, is an essential light chain of myosin that is associated with muscle contraction. It is expressed in ventricular and slow skeletal muscle. MYL3 may serve as a target for caspase-3 in dying cardiomyocytes. Mutations of MYL3 gene cause hypertrophic cardiomyopathy. MYL3 has been identified as potential serum biomarker for drug induced myotoxicity. Great increase in MYL3 serum concentration has been observed in rats with cardiac and skeletal muscle injury. (PMID:21685905)

Publications

Species	Application	Title
human	WB	Nat Biotechnol; A pipeline that integrates the discovery and verification of plasma protein biomarkers reveals candidate markers for cardiovascular disease.; Authors - Addona Terri A TA
	WB	J Proteome Res; Multi-Proteomic Analysis Reveals the Effect of Protein Lactylation on Matrix and Cholesterol Metabolism in Tendinopathy; Authors - Yuan Lin
mouse,human	WB,IHC,IF	Nat Commun; MYL3 protects chondrocytes from senescence by inhibiting clathrin-mediated endocytosis and activating of Notch signaling; Authors - He Cao
mouse	WB	Nat Commun; Systematic HOIP interactome profiling reveals critical roles of linear ubiquitination in tissue homeostasis; Authors - Yesheng Fu

Produits associés

Flow Cytometry Perm Buffer (10X)

PF00011-C