Anticorps Polyclonal de lapin anti-MYL12A

MYL12A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 16287-1-AP

Synonymes

MLC 2B, MLCB, MRLC3, MYL12A, MYL2B, Myosin RLC, RLC

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Galerie de données de validation

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  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 16287-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 16287-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 16287-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human skeletal muscle using 16287-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse bladder using 16287-1-AP

  mouse bladder tissue were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of NIH/3T3 using 16287-1-AP

  NIH/3T3 cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Jurkat using 16287-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 16287-1-AP (MYL12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 16287-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 16287-1-AP (MYL12A Antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules HEK-293, cellules HeLa, cellules Jurkat, tissu cardiaque de souris, tissu de muscle squelettique humain, tissu vésical de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

16287-1-AP cible MYL12A dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MYL12A Protéine recombinante Ag9413
• Nom complet: myosin, light chain 12A, regulatory, non-sarcomeric
• Masse moléculaire calculée: 171 aa, 20 kDa
• Poids moléculaire observé: 19-20 kDa
• Numéro d’acquisition GenBank: BC016372
• Symbole du gène: MYL12A
• Identification du gène (NCBI): 10627
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
pig	WB	Biochem J; Kinase-related protein/telokin inhibits Ca2+-independent contraction in Triton-skinned guinea pig taenia coli.; Authors - Shcherbakova Olga V OV
mouse	WB	Front Physiol; Distinct Roles of Smooth Muscle and Non-muscle Myosin Light Chain-Mediated Smooth Muscle Contraction.; Authors - Jie Sun
human	IHC	Biofizika; [Novel peptide inhibitors of the myosin light chain kinase suppress hyperpermeability of vascular endothelium].; Authors - Marchenko A V AV
mouse	WB	EMBO J; Salmonella manipulates macrophage migration via SteC-mediated myosin light chain activation to penetrate the gut-vascular barrier; Authors - Yuanji Dai