Anticorps Polyclonal de lapin anti-MYH6

• Phare
• Validé par KD/KO

MYH6 Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IHC, IF-P, ELISA

Conjugaison

Non conjugué

Publications

40

Avis (3)

N° de cat : 22281-1-AP

Synonymes

alpha MHC, ASD3, MYH6, MYHC, MyHC alpha, MYHCA, Myosin 6, Myosin heavy chain 6

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Galerie de données de validation

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  WB analysis using 22281-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22281-1-AP (MYH6 antibody) at dilution of 1:25000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 22281-1-AP

  IP result of anti-MYH6 (IP:22281-1-AP, 5ug; Detection:22281-1-AP 1:300) with mouse heart tissue lysate 4000ug.

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  IHC staining of human heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of mouse heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of mouse heart using 22281-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 22281-1-AP (MYH6 Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse heart using 22281-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse heart tissue using 22281-1-AP (MYH6 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cœur de rat
• Résultats positifs en IHC: tissu cardiaque humain, tissu cardiaque de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cardiaque de souris

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 24 publications below
• IHC: See 4 publications below
• IF: See 12 publications below

Informations sur le produit

22281-1-AP cible MYH6 dans les applications de WB, IHC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: myosin, heavy chain 6, cardiac muscle, alpha
• Masse moléculaire calculée: 224 kDa
• Poids moléculaire observé: 200-220 kDa
• Numéro d’acquisition GenBank: NM_002471
• Symbole du gène: MYH6
• Identification du gène (NCBI): 4624
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYH6 encodes the alpha-cardiac myosin heavy chain (aMHC) which is a major contractile protein exclusively expressed in the myocardium of the heart. Expression of MYH6 is down-regulated in cardiac myopathy and failing hearts. Defects in MYH6 lead to congenital heart disease (CHD), and a spectrum of phenotypes ranging from hypertrophic cardiomyopathy (HCM) and dilated cardiomyopathy (DCM). (15737621, 20656787)

Publications

Species	Application	Title
human	WB	Science; HEART DISEASE. Titin mutations in iPS cells define sarcomere insufficiency as a cause of dilated cardiomyopathy.; Authors - John T Hinson
		Dev Cell; Force Generation via β-Cardiac Myosin, Titin, and α-Actinin Drives Cardiac Sarcomere Assembly from Cell-Matrix Adhesions.; Authors - Anant Chopra
human	WB	Elife; Independent regulation of Z-lines and M-lines during sarcomere assembly in cardiac myocytes revealed by the automatic image analysis software sarcApp; Authors - Abigail C Neininger-Castro ; KD Validated
human	IF	Stem Cell Reports; Distinctive Roles of Canonical and Noncanonical Wnt Signaling in Human Embryonic Cardiomyocyte Development.; Authors - Silvia Mazzotta
mouse		J Pathol; Doxorubicin-induced cardiotoxicity involves IFNγ-mediated metabolic reprogramming in cardiomyocytes.; Authors - Chen Ni
chicken	WB,IF	Int J Mol Sci; Circular RNA circIGF2BP3 Promotes the Proliferation and Differentiation of Chicken Primary Myoblasts; Authors - Xiaotong Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (01-20-2023)

Very good antibody. Strong signal and clean background in WB.

• Applications: Western Blot
• Primary Antibody Dilution: 1:8000
• Cell Tissue Type: Mouse heart

MYH6 Antibody Western Blot validation (1:8000 dilution) in Mouse heart (Cat no:22281-1-AP)

FH

Jane (Verified Customer) (02-03-2022)

one specific band around 230kDa, however, the band was not very sharp. Probably will help if run at a lower percentage gel.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: Heart tissue

FH

Jing (Verified Customer) (02-08-2021)

good antibody, clear sigle band around 260KD.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse heart