Anticorps Polyclonal de lapin anti-MYC tag
MYC tag Polyclonal Antibody for WB, IP, IF, ELISA
Hôte / Isotype
Lapin / IgG
Réactivité testée
Protéine recombinante et plus (3)
Applications
WB, IHC, IF/ICC, IP, CoIP, ELISA
Conjugaison
Non conjugué
337
N° de cat : 16286-1-AP
Synonymes
"MYC tag Antibodies" Comparison
View side-by-side comparison of MYC tag antibodies from other vendors to find the one that best suits your research needs.
Applications testées
| Résultats positifs en WB | protéine protéine recombinante, Protéine recombinante |
| Résultats positifs en IP | cellules HEK-293 transfectées, |
| Résultats positifs en IF/ICC | cellules HEK-293 transfectées, cellules MCF-7 |
Dilution recommandée
| Application | Dilution |
|---|---|
| Western Blot (WB) | WB : 1:1000-1:8000 |
| Immunoprécipitation (IP) | IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate |
| Immunofluorescence (IF)/ICC | IF/ICC : 1:200-1:800 |
| It is recommended that this reagent should be titrated in each testing system to obtain optimal results. | |
| Sample-dependent, check data in validation data gallery | |
Informations sur le produit
16286-1-AP cible MYC tag dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Protéine recombinante
| Réactivité | Protéine recombinante |
| Réactivité citée | Humain, poulet, souris |
| Hôte / Isotype | Lapin / IgG |
| Clonalité | Polyclonal |
| Type | Anticorps |
| Immunogène | MYC tag Protéine recombinante Ag9409 |
| Nom complet | MYC tag |
| Symbole du gène | |
| Identification du gène (NCBI) | |
| Conjugaison | Non conjugué |
| Forme | Liquide |
| Méthode de purification | Purification par affinité contre l'antigène |
| Tampon de stockage | PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3 |
| Conditions de stockage | Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA. |
Informations générales
The myc-tag is a short synthetic polypeptide sequence derived from c-myc protein that can be added to recombinant proteins to enable isolation and study when an antibody is not available. This antibody recognizes the MYC tag EQKLISEEDL.
What is the molecular weight of myc?
1203Da: the ten amino acid myc tag sequence is EQKLISEEDL (in single letter code).
What are the applications for myc tag?
The addition of the myc tag to a particular protein can be useful when an antibody is not available to the protein of interest. Using recombinant DNA technology, the myc tag can be fused to the protein and then an antibody against the myc tag can be used to probe (PMID: 24490106). This is a reliable method and can be used in a number of different techniques, including purification using chromatography, tracking the protein in localization studies using immunofluorescence, or quantifying levels using Western Blot (PMID: 24490106).
What is the structure of myc tag?
The c-myc gene from which this tag is derived has a molecular weight of 49kDa, but the myc tag represents only a small portion of the C-terminus of this gene. The short polypeptide sequence can be fused to the N-terminus or the C-terminus of any protein without influencing function, although it is advised to avoid fusing it to a secretory signal. A cleavage site behind this tag is also sometimes engineered to allow removal with a specific protease.
Protocole
| Product Specific Protocols | |
|---|---|
| WB protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IF protocol for MYC tag antibody 16286-1-AP | Download protocol |
| IP protocol for MYC tag antibody 16286-1-AP | Download protocol |
| Standard Protocols | |
|---|---|
| Click here to view our Standard Protocols |
Publications
| Species | Application | Title |
|---|---|---|
Signal Transduct Target Ther TRAF3 activates STING-mediated suppression of EV-A71 and target of viral evasion | ||
Nat Microbiol Ephrin receptor A2 is an epithelial cell receptor for Epstein-Barr virus entry. | ||
Nat Commun TRIM5α recruits HDAC1 to p50 and Sp1 and promotes H3K9 deacetylation at the HIV-1 LTR | ||
Nat Commun The CUL5 E3 ligase complex negatively regulates central signaling pathways in CD8+ T cells | ||
Mol Cell CPT1A induction following epigenetic perturbation promotes MAVS palmitoylation and activation to potentiate antitumor immunity | ||
Mol Cell Lactylation-driven METTL3-mediated RNA m6A modification promotes immunosuppression of tumor-infiltrating myeloid cells. |
Avis
The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.
FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot, IF, IP
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FH Nazarine (Verified Customer) (02-01-2024) | Works well for Western Blot and IF and IP
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FH Amy (Verified Customer) (03-05-2023) | Detected overexpressed Mac-tagged proteins in HEK293T cell lysates but with a fair few background bands.
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FH James (Verified Customer) (11-30-2022) | Very good antibody
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FH Boyan (Verified Customer) (08-03-2022) | gives a good signal in IF
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FH Chahat (Verified Customer) (06-08-2022) | The myc antibody gives clear staining!
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FH WEI (Verified Customer) (03-08-2022) | Good antibody for detecting overexpression proteins in 293 cells.
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FH Minze (Verified Customer) (01-11-2022) | it works well for immunofluorecence so far.
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FH Phoebe (Verified Customer) (03-01-2019) | This antibody was very clean in a western blot to detect Myc-tagged pol2 in S cerevisiae whole cell extract and chromatin fractionation samples.
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