Anticorps Recombinant de lapin anti-c-MYC

• Phare
• Validé par KD/KO

c-MYC Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR

Conjugaison

Non conjugué

CloneNo.

2B17

Avis (1)

N° de cat : 80845-1-RR

Synonymes

MYC, 2B17, bHLHe39, c Myc, Class E basic helix-loop-helix protein 39

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Galerie de données de validation

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  WB analysis of A549 using 80845-1-RR

  WB result of MYC antibody (80845-1-RR; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-MYC transfected A549 cells.

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  WB analysis using 80845-1-RR

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 80845-1-RR (c-MYC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 80845-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 80845-1-RR (MYC antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse embryo using 80845-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse embryo tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 80845-1-RR

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 80845-1-RR (c-MYC antibody) at dilution of 1:400 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 80845-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  IF Staining of HEK-293 using 80845-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HEK-293 cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of U2OS using 80845-1-RR

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using MYC antibody (80845-1-RR, Clone: 2B17 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  FC experiment of HeLa using 80845-1-RR

  1x10^6 HeLa cells were intracellularly stained with 0.25 ug c-MYC Recombinant antibody (80845-1-RR, Clone:2B17) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

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  ChIP experiment of HeLa using 80845-1-RR

  Chromatin was prepared from HeLa cells. Cells were fixed with formaldehyde for 10 minutes. The ChIP was performed with 20 µg of cross-linked chromatin, 5 µg of c-MYC (80845-1-RR) or 5 ug of Normal Rabbit IgG (98136-1-RR), and 20 µl of Protein A Magarose Beads. The immunoprecipitated DNA was quantified by real-time PCR.

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  Affinity and Kinetic Characterization of 80845-1-RR

  Biolayer interferometry (BLl) kinetic assays of 80845-1-RR against Human c-MYC were performed. The affinity constant is 2.03 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A549, cellules HeLa, cellules HL-60, cellules K-562
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu embryonnaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa, cellules HEK-293, cellules U2OS
• Résultats positifs en FC (Intra): cellules HeLa,
• Résultats positifs en ChIP-qPCR: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension
• CHImmunoprécipitation (IP)-QPCR: CHIP-QPCR : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

80845-1-RR cible c-MYC dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA, ChIP-qPCR et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: c-MYC Protéine recombinante Ag1263
• Nom complet: v-myc myelocytomatosis viral oncogene homolog (avian)
• Masse moléculaire calculée: 49 kDa
• Poids moléculaire observé: 50-65 kDa
• Numéro d’acquisition GenBank: BC000141
• Symbole du gène: MYC
• Identification du gène (NCBI): 4609
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MYC contains one basic helix-loop-helix (bHLH) domain. This protein is a multifunctional, nuclear phosphoprotein that plays a role in cell cycle progression, apoptosis and cellular transformation. It functions as a transcription factor that regulates transcription of specific target genes. It seems to activate the transcription of growth-related genes. It binds DNA in a non-specific manner, yet also specifically recognizes the core sequence 5'-CAC[GA]TG-3'. Mutations, overexpression, rearrangement and translocation of this gene have been associated with a variety of hematopoietic tumors, leukemias and lymphomas, including Burkitt lymphoma.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nikolett (Verified Customer) (07-29-2025)

Antibody works well for western blot in 1:1000 dilution, incubated overnight in 3%BSA/PBS on human lung cancer cell lines and mouse lung cancer cell lines. For IHC in a 1:300 dilution upon pH6 NaCi based antigene retrieval it gives a specific nuclear signal in human lung cancer patient derived samples as well as on murine lung cancer tissue.

• Applications: Western Blot, Immunohistochemistry
• Primary Antibody Dilution: 1:1000 for Western Blot, 1:300 for IHC
• Cell Tissue Type: Human Lung Cancer Patient Samples; Murine Lung Cancer Tissue Samples (FFPE embedded)

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