Anticorps Polyclonal de lapin anti-MVD

MVD Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (1)

N° de cat : 15331-1-AP

Synonymes

FP17780, MDDase, MPD, MVD

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Galerie de données de validation

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  WB analysis of HepG2 using 15331-1-PBS

  HepG2 cells were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 15331-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat liver using 15331-1-AP

  rat liver tissue were subjected to SDS PAGE followed by western blot with 15331-1-AP (MVD Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15331-1-AP (MVD antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human lung cancer using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 15331-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 15331-1-AP (MVD Antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of A431 using 15331-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed A431 cells using 15331-1-AP (MVD antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HepG2, cellules K-562, tissu hépatique de rat
• Résultats positifs en IHC: tissu de côlon humain, tissu cardiaque humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 1 publications below

Informations sur le produit

15331-1-AP cible MVD dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MVD Protéine recombinante Ag7255
• Nom complet: mevalonate (diphospho) decarboxylase
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 66-74 kDa, 45 kDa, 37 kDa
• Numéro d’acquisition GenBank: BC000011
• Symbole du gène: MVD
• Identification du gène (NCBI): 4597
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The enzyme mevalonate pyrophosphate decarboxylase(MVD) catalyzes the conversion of mevalonate pyrophosphate into isopentenyl pyrophosphate. It is also named as MPD and as a unique enzyme in one of the early steps in cholesterol biosynthesis, MVD may be a useful target for drugs aimed at lowering serum cholesterol levels(PMID:8626466). The intracellular glycosylation does not contribute to the difference between the 45 and 37 kDa species of MVD. The native MVD has a molecular weight of 90 kDa that it consists of two identical subunits of 45 kDa and a 37 kDa protein is also found as a subunit of MVD and this type of MVD may be a 74 kDa.But the 37 kDa enzyme appeared only when the rats are fed the CP diet.(PMID:9348097).

Publications

Species	Application	Title
human	WB	J Hepatocell Carcinoma; miR-612 Enhances RSL3-Induced Ferroptosis of Hepatocellular Carcinoma Cells via Mevalonate Pathway; Authors - Kailin Xing
mouse	IHC	Cell Signal; Circ_0011058 facilitates proliferation, angiogenesis and radioresistance in papillary thyroid cancer cells by positively regulating YAP1 via acting as miR-335-5p sponge.; Authors - Zhenhua Zhang
mouse	WB	Curr Issues Mol Biol; Gigantol Improves Cholesterol Metabolism and Progesterone Biosynthesis in MA-10 Leydig Cells.; Authors - Audrey Basque
human	WB	Front Pharmacol; CCR9 overexpression promotes T-ALL progression by enhancing cholesterol biosynthesis; Authors - Muhammad Jamal

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

k (Verified Customer) (06-10-2021)

We always got good bands in western with this Ab

• Applications: Western Blot
• Primary Antibody Dilution: 1;1000