Anticorps Polyclonal de lapin anti-MTPN

MTPN Polyclonal Antibody for WB, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 13508-1-AP

Synonymes

GCDP, MTPN, myotrophin, Protein V 1, V 1

---

Galerie de données de validation

• 

  WB analysis using 13508-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 13508-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13508-1-AP (MTPN antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human cerebellum using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

• 

  IHC staining of human heart using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50.

• 

  IHC staining of human cerebellum using 13508-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 13508-1-AP (MTPN Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of HepG2 using 13508-1-AP

  Immunofluorescent analysis of HepG2 cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HeLa using 13508-1-AP

  Immunofluorescent analysis of Hela cells, using MTPN antibody 13508-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

---

Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules HeLa, cellules Jurkat, tissu cérébral humain
• Résultats positifs en IHC: tissu de cervelet humain, tissu cardiaque humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 2 publications below

Informations sur le produit

13508-1-AP cible MTPN dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTPN Protéine recombinante Ag4419
• Nom complet: myotrophin
• Masse moléculaire calculée: 118 aa, 13 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC028093
• Symbole du gène: MTPN
• Identification du gène (NCBI): 136319
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Myotrophin (MTPN) is a ubiquitously expressed 12 kDa cytoplamic protein that was first isolated from the hearts of spontaneously hypertensive rats. Protein sequence analysis revealed that MTPN was composed of 118 amino acids and was occupied by two and a half internal 33 amino acid ankyrin repeats. MTPN stimulates protein synthesis and increases the expression of a number of cardiac marker genes (such as atrial natriuretic factor and b-myosin heavy chain) in cardiomyocytes leading to hypertrophy. In skeletal muscle, MTPN is also a positive growth factor in promoting skeletal muscle growth.

Publications

Species	Application	Title
human	IHC	Am J Obstet Gynecol; Trophoblast apoptosis from pregnancies complicated by fetal growth restriction is associated with enhanced p53 expression.; Authors - Roni Levy
human	IHC	J Clin Biochem Nutr; Upregulation of ENDOU in cytotrophoblasts from placenta complicated with preeclampsia and fetal growth restriction.; Authors - Masataka Nomoto