Anticorps Polyclonal de lapin anti-MTMR2

• Phare
• Validé par KD/KO

MTMR2 Polyclonal Antibody for WB, IP, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Avis (1)

N° de cat : 14372-1-AP

Synonymes

CMT4B, CMT4B1, KIAA1073, MTMR2, myotubularin related protein 2

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Galerie de données de validation

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  WB analysis using 14372-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 14372-1-AP

  WB result of MTMR2 antibody (14372-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-MTMR2 transfected HeLa cells.

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  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of human cerebellum using 14372-1-AP

  human cerebellum tissue were subjected to SDS PAGE followed by western blot with 14372-1-AP (MTMR2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse cerebellum using 14372-1-AP

  IP result of anti-MTMR2 (IP:14372-1-AP, 4ug; Detection:14372-1-AP 1:300) with mouse cerebellum tissue lysate 4000 ug.

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  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human endometrial cancer using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human cerebellum using 14372-1-AP

  Immunohistochemical analysis of paraffin-embedded human cerebellum tissue slide using 14372-1-AP (MTMR2 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules NIH/3T3, tissu de cervelet humain
• Résultats positifs en IP: tissu de cervelet de souris,
• Résultats positifs en IHC: tissu de cancer de l'endomètre humain, tissu de cervelet humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:400

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14372-1-AP cible MTMR2 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTMR2 Protéine recombinante Ag5745
• Nom complet: myotubularin related protein 2
• Masse moléculaire calculée: 73 kDa
• Poids moléculaire observé: 66-73 kDa
• Numéro d’acquisition GenBank: BC052990
• Symbole du gène: MTMR2
• Identification du gène (NCBI): 8898
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTMR2(myotubularin-related protein 2) is also named as KIAA1073 and belongs to the non-receptor class myotubularin subfamily, which is characterized by the presence of a phosphatase domain. It may regulate vesicular trafficking in Schwann cells (PMID:17880751) and its reversible phosphorylation represents a critical mechanism for regulating the subcellular localization of MTMR2(PMID:21372139). MTMR2 is a 73 kDa protein in its monomeric form and exist as a dimer within cells and the protein re-localizes to membranes in hypo-osmotic settings(PMID:16399794). Defects in MTMR2 are the cause of Charcot-Marie-Tooth disease type 4B1 (CMT4B1). MTMR2 has two isofroms with the molecular mass of 73 and 66 kDa.

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Kincaid (Verified Customer) (05-21-2024)

This product worked as expected. I completed a GFP-nanotrap IP (Chromotek agarose beads) and ran 2% whole cell lysate with IP fraction on an SDS-PAGE gel. Resolved proteins were transferred to a PVDF membrane and probed with aMTMR2. GFP-CCDC22 is known to pull-down MTMR2 and is validated with this antibody.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T

MTMR2 Antibody Western Blot validation (1:1000 dilution) in HEK293T (Cat no:14372-1-AP)