Anticorps Polyclonal de lapin anti-MTMR15

• Phare
• Validé par KD/KO

MTMR15 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

3

Avis (1)

N° de cat : 17600-1-AP

Synonymes

FAN1, Fanconi associated nuclease 1, KIAA1018, MTMR15

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Galerie de données de validation

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  WB analysis using 17600-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human spleen using 17600-1-AP

  human spleen tissue were subjected to SDS PAGE followed by western blot with 17600-1-AP (MTMR15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 17600-1-AP

  IP result of anti-MTMR15 (IP:17600-1-AP, 4ug; Detection:17600-1-AP 1:300) with HeLa cells lysate 2800 ug.

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Applications testées

• Résultats positifs en WB: cellules HeLa, tissu splénique humain
• Résultats positifs en IP: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 3 publications below
• IP: See 1 publications below

Informations sur le produit

17600-1-AP cible MTMR15 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTMR15 Protéine recombinante Ag11651
• Nom complet: myotubularin related protein 15
• Masse moléculaire calculée: 60 kDa, 114 kDa
• Poids moléculaire observé: 63-68 kDa, 120-140 kDa
• Numéro d’acquisition GenBank: BC047882
• Symbole du gène: MTMR15
• Identification du gène (NCBI): 22909
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

MTMR15, also named as FAN1 and KIAA1018, is a nuclease required for the repair of DNA interstrand cross-links (ICL) recruited at sites of DNA damage by monoubiquitinated FANCD2. It is specifically involved in repair of ICL-induced DNA breaks by being required for efficient homologous recombination, probably in the resolution of homologous recombination intermediates. MTMR15 has some isoforms with MW 114 kDa and 60 kDa. The MW will be migrated to 120-140 kDa and 63-68 kDa with modification.

Publications

Species	Application	Title
	WB	Nat Struct Mol Biol; Fanconi anemia proteins participate in a break-induced-replication-like pathway to counter replication stress.; Authors - Xinlin Xu
human	WB,IP	Genes Cells; MLH1-mediated recruitment of FAN1 to chromatin for the induction of apoptosis triggered by O6 -methylguanine.; Authors - Mihoko Rikitake ; KD Validated
human	WB	Cell Rep; HIV-1 exploits the Fanconi anemia pathway for viral DNA integration.; Authors - Shaozu Fu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Alexandra (Verified Customer) (07-04-2024)

Detectable both with endogenous and overexpressed protein from plasmid. One of the two bands ran in between the two expected molecular weights, the other was at the proper size. It is usable for the examination of the effect of endogenous gene silencing and for overexpression as well. Possibly higher dilution and longer incubation with the antibody is enough.

• Applications: Western Blot, Immunofluorescence, Cell culture
• Primary Antibody Dilution: 1000x
• Cell Tissue Type: U2OS human osteosarcoma cell line

MTMR15 Antibody Western Blot,Immunofluorescence,Cell culture validation (1000x dilution) in U2OS human osteosarcoma cell line (Cat no:17600-1-AP)