Anticorps Polyclonal de lapin anti-MTMR12

MTMR12 Polyclonal Antibody for WB, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 17552-1-AP

Synonymes

3 PAP, 3 phosphatase adapter protein, KIAA1682, MTMR12, PIP3AP

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Galerie de données de validation

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  WB analysis using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 17552-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse lung using 17552-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 17552-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 17552-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 17552-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 17552-1-AP (MTMR12 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of K-562 using 17552-1-AP

  IP result of anti-MTMR12 (IP:17552-1-AP, 4ug; Detection:17552-1-AP 1:500) with K-562 cells lysate 2400 ug.

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, tissu cérébral de souris, tissu pulmonaire de souris
• Résultats positifs en IP: cellules K-562,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IP: See 1 publications below

Informations sur le produit

17552-1-AP cible MTMR12 dans les applications de WB, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTMR12 Protéine recombinante Ag11710
• Nom complet: myotubularin related protein 12
• Masse moléculaire calculée: 86 kDa
• Poids moléculaire observé: 73 kDa
• Numéro d’acquisition GenBank: BC057393
• Symbole du gène: MTMR12
• Identification du gène (NCBI): 54545
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB,IP	FASEB Bioadv; In search of alternatively spliced alpha-Klotho Kl1 protein in mouse brain; Authors - Liping Li