Anticorps Polyclonal de lapin anti-MTHFD2

• Phare
• Validé par KD/KO

MTHFD2 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications

41

Avis (2)

N° de cat : 12270-1-AP

Synonymes

MTHFD2

---

Galerie de données de validation

• 

  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 12270-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12270-1-AP (MTHFD2 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of A549 using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:4000) with A549 cells lysate 1480 ug.

• 

  IP experiment of mouse testis using 12270-1-AP

  IP result of anti-MTHFD2 (IP:12270-1-AP, 4ug; Detection:12270-1-AP 1:300) with mouse testis tissue lysate 4000ug.

• 

  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 12270-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12270-1-AP (MTHFD2 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HepG2 using 12270-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using MTHFD2 antibody (12270-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

---

Applications testées

• Résultats positifs en WB: cellules A549, cellules HCT 116, cellules HEK-293T, cellules Jurkat, tissu testiculaire de rat, tissu testiculaire de souris
• Résultats positifs en IP: cellules A549, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:200-1:800
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 36 publications below
• IHC: See 4 publications below
• IF: See 3 publications below
• IP: See 2 publications below
• CoIP: See 1 publications below

Informations sur le produit

12270-1-AP cible MTHFD2 dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: MTHFD2 Protéine recombinante Ag2911
• Nom complet: methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase
• Masse moléculaire calculée: 350 aa, 38 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC017054
• Symbole du gène: MTHFD2
• Identification du gène (NCBI): 10797
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) is a folate-coupled mitochondrial metabolic enzyme characterized by methylenetetrahydrofolate dehydrogenase and cyclohydrolase activity. It was demonstrated that suppression of MTHFD2 inhibits methylation reactions, decreases protein synthesis and disrupts redox homeostasis, which may ultimately result in significant changes in cellular metabolic phenotype. MTHFD2 is reported to be up‐regulated in various tumours, such as lung adenocarcinoma(PMID: 34121323) and breast cancer(PMID: 34007312).

Publications

Species	Application	Title
human	WB	Nature; Oxidative stress inhibits distant metastasis by human melanoma cells.; Authors - Elena Piskounova
mouse	WB	Science; mTORC1 induces purine synthesis through control of the mitochondrial tetrahydrofolate cycle.; Authors - Issam Ben-Sahra
human	WB	Cancer Cell; Nrf2 redirects glucose and glutamine into anabolic pathways in metabolic reprogramming.; Authors - Yoichiro Mitsuishi
human	WB	Cell Metab; Epstein-Barr-Virus-Induced One-Carbon Metabolism Drives B Cell Transformation.; Authors - Liang Wei Wang
mouse	WB	Cell Metab; Perturbed Redox Signaling Exacerbates a Mitochondrial Myopathy.; Authors - Sukru Anil Dogan
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

---

FH

Ayse Tarbin (Verified Customer) (03-16-2023)

It was a sample gift antibody. It has a band at corresponding kDa but there are also unspesific bands just bellow 30 kDa.

• Applications: Western Blot, Cell culture
• Primary Antibody Dilution: 1:2000 in 5% milk in TBST
• Cell Tissue Type: Mouse satellite cells

MTHFD2 Antibody Western Blot,Cell culture validation (1:2000 in 5% milk in TBST dilution) in Mouse satellite cells (Cat no:12270-1-AP)

FH

Hua (Verified Customer) (10-21-2020)

There is no band at the expected size. There is a strong non-specific band at 100 kDa.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver lysate

MTHFD2 Antibody Western Blot validation (1:500 dilution) in mouse liver lysate (Cat no:12270-1-AP)